检测流感 H7N9 病毒：并非所有分子检测都一样。
Detection of influenza H7N9 virus: all molecular tests are not equal.
机构信息
Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority (CDHA), Halifax, Nova Scotia, Canada.
出版信息
J Clin Microbiol. 2013 Nov;51(11):3835-8. doi: 10.1128/JCM.01808-13. Epub 2013 Aug 21.
Abstract
The recent emergence of influenza A virus (H7N9) emphasizes the need for its rapid detection. While commercial nucleic acid amplification tests (NAATs) are commonly used to detect seasonal influenza virus, this study demonstrated that the analytical sensitivity of commercial assays is highly variable compared to that of CDC-based in-house NAATs for the detection of H7N9.
摘要
最近甲型流感病毒（H7N9）的出现强调了其快速检测的必要性。虽然商业核酸扩增检测（NAAT）通常用于检测季节性流感病毒，但本研究表明，与基于疾控中心的内部 NAAT 相比，商业检测在检测 H7N9 方面的分析灵敏度具有高度可变性。
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2
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Euro Surveill. 2013 May 23;18(21):20487.
3
Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China.
Euro Surveill. 2013 Apr 18;18(16):20461.
4
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N Engl J Med. 2013 May 16;368(20):1888-97. doi: 10.1056/NEJMoa1304459. Epub 2013 Apr 11.
5
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Influenza Other Respir Viruses. 2013 Jul;7(4):491-6. doi: 10.1111/irv.12017. Epub 2012 Sep 18.
6
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Crit Rev Clin Lab Sci. 2011 Sep-Dec;48(5-6):217-49. doi: 10.3109/10408363.2011.640976.
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J Clin Microbiol. 2009 Dec;47(12):3805-13. doi: 10.1128/JCM.01344-09. Epub 2009 Sep 30.
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