基于实时 PCR 的加拿大实验室中 SARS-CoV-2 的检测。
Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories.
机构信息
Division of Microbiology, Department of Pathology and Laboratory Medicine, Nova Scotia Health Authority (NSHA), Room 404B, MacKenzie Building, 5788 University Avenue, Halifax, Nova Scotia B3H 1V8, Canada; Departments of Pathology, Medicine, and Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.
Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Public Health Ontario Laboratories, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada.
出版信息
J Clin Virol. 2020 Jul;128:104433. doi: 10.1016/j.jcv.2020.104433. Epub 2020 May 13.
Abstract
With emergence of pandemic COVID-19, rapid and accurate diagnostic testing is essential. This study compared laboratory-developed tests (LDTs) used for the detection of SARS-CoV-2 in Canadian hospital and public health laboratories, and some commercially available real-time RT-PCR assays. Overall, analytical sensitivities were equivalent between LDTs and most commercially available methods.
摘要
随着大流行 COVID-19 的出现，快速准确的诊断测试至关重要。本研究比较了加拿大医院和公共卫生实验室使用的用于检测 SARS-CoV-2 的实验室开发的检测方法（LDT）和一些市售的实时 RT-PCR 检测方法。总体而言，LDT 与大多数市售方法的分析灵敏度相当。
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