UPMC University of Paris, Paris, France.
J Clin Microbiol. 2013 Nov;51(11):3616-23. doi: 10.1128/JCM.01714-13. Epub 2013 Aug 21.
The complete 154-kbp linear double-stranded genomic DNA sequence of herpes simplex virus 2 (HSV-2), consisting of two extended regions of unique sequences bounded by a pair of inverted repeat elements, was published in 1998 and since then has been widely employed in a wide range of studies. Throughout the HSV-2 genome are scattered 150 microsatellites (also referred to as short tandem repeats) of 1- to 6-nucleotide motifs, mainly distributed in noncoding regions. Microsatellites are considered reliable markers for genetic mapping to differentiate herpesvirus strains, as shown for cytomegalovirus and HSV-1. The aim of this work was to characterize 12 polymorphic microsatellites within the HSV-2 genome by use of 3 multiplex PCR assays in combination with length polymorphism analysis for the rapid genetic differentiation of 56 HSV-2 clinical isolates and 2 HSV-2 laboratory strains (gHSV-2 and MS). This new system was applied to a specific new HSV-2 variant recently identified in HIV-1-infected patients originating from West Africa. Our results confirm that microsatellite polymorphism analysis is an accurate tool for studying the epidemiology of HSV-2 infections.
单纯疱疹病毒 2（HSV-2）的完整 154-kbp 线性双链基因组 DNA 序列于 1998 年公布，由一对反向重复元件所限定的两个扩展的独特序列区组成，此后被广泛应用于广泛的研究中。在 HSV-2 基因组中散布着 150 个 1-6 核苷酸基序的微卫星（也称为短串联重复），主要分布在非编码区域。微卫星被认为是遗传图谱标记，用于区分疱疹病毒株，例如巨细胞病毒和 HSV-1。本工作的目的是通过使用 3 个多重 PCR 分析结合长度多态性分析，对 56 个 HSV-2 临床分离株和 2 个 HSV-2 实验室株（gHSV-2 和 MS）中的 12 个多态性微卫星进行特征描述，从而快速区分 HSV-2 基因。该新系统应用于最近在来自西非的 HIV-1 感染患者中鉴定的特定新的 HSV-2 变体。我们的结果证实，微卫星多态性分析是研究 HSV-2 感染的流行病学的一种准确工具。
