Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, P. R. China.
PLoS One. 2013 Aug 14;8(8):e70986. doi: 10.1371/journal.pone.0070986. eCollection 2013.
The moderate halophile Amycolicicoccus subflavus DQS3-9A1(T) is the type strain of a novel species in the recently described novel genus Amycolicicoccus, which was isolated from oil mud precipitated from oil produced water. The complete genome of A. subflavus DQS3-9A1(T) has been sequenced and is characteristic of harboring the genes for adaption to the harsh petroleum environment with salinity, high osmotic pressure, and poor nutrient levels. Firstly, it characteristically contains four types of alkane hydroxylases, including the integral-membrane non-heme iron monooxygenase (AlkB) and cytochrome P450 CYP153, a long-chain alkane monooxygenase (LadA) and propane monooxygenase. It also accommodates complete pathways for the response to osmotic pressure. Physiological tests proved that the strain could grow on n-alkanes ranging from C10 to C36 and propane as the sole carbon sources, with the differential induction of four kinds of alkane hydroxylase coding genes. In addition, the strain could grow in 1-12% NaCl with the putative genes responsible for osmotic stresses induced as expected. These results reveal the effective adaptation of the strain DQS3-9A1(T) to harsh oil environment and provide a genome platform to investigate the global regulation of different alkane metabolisms in bacteria that are crucially important for petroleum degradation. To our knowledge, this is the first report to describe the co-existence of such four types of alkane hydroxylases in a bacterial strain.
中度嗜盐菌 Amycolicicoccus subflavus DQS3-9A1(T) 是最近描述的新型 Amycolicicoccus 属中一个新型物种的模式菌株,它是从采油污水产生的油泥中分离出来的。A. subflavus DQS3-9A1(T) 的全基因组已测序,其特征是含有适应高盐度、高渗透压和低营养水平的石油环境的基因。首先,它特异地包含四种烷烃羟化酶,包括整合膜非血红素铁单加氧酶 (AlkB) 和细胞色素 P450 CYP153、长链烷烃单加氧酶 (LadA) 和丙烷单加氧酶。它还容纳了对渗透压响应的完整途径。生理测试证明,该菌株可以以 n-烷烃(从 C10 到 C36)和丙烷作为唯一碳源生长,并对四种烷烃羟化酶编码基因进行差异诱导。此外,该菌株可以在 1-12%的 NaCl 中生长,预期与渗透压应激相关的假定基因被诱导。这些结果揭示了菌株 DQS3-9A1(T) 对恶劣石油环境的有效适应,并为研究细菌中不同烷烃代谢的全局调控提供了基因组平台,这对石油降解至关重要。据我们所知,这是首次描述在一个细菌菌株中同时存在这四种类型的烷烃羟化酶。