Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou 450008, PR China.
Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing 100871, PR China.
Int J Syst Evol Microbiol. 2010 Mar;60(Pt 3):638-643. doi: 10.1099/ijs.0.010546-0. Epub 2009 Aug 4.
Two novel actinomycetes, designated DQS3-9A1(T) and DQS3-9A2, were isolated from a saline soil contaminated with crude oil in the Shengli Oilfield in China. On the basis of 16S rRNA gene sequence analysis, the two strains were most closely related to Mycobacterium species (92.7-94.9 % similarities), and formed a distinct lineage in the suborder Corynebacterineae . In addition, the major sugars in the cell wall, arabinose and galactose, supported the affiliation of strain DQS3-9A1(T) with members of the family Mycobacteriaceae. However, strain DQS3-9A1(T) did not contain mycolic acids and MK-8 (85.5 %) was the major menaquinone for both isolates. The major cellular fatty acids for strain DQS3-9A1(T) were C(16 : 0) (20.5 %), 10-methyl C(17 : 0) (19.3 %), 10-methyl C(18 : 0) (16.1 %), summed feature 3 (11.4 %), C(15 : 0) (11.3 %), C(17 : 0) (5.0 %) and C(17 : 1)omega8c (5.0 %). The polar lipids of strain DQS3-9A1(T) consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unknown glucosamine-containing phospholipid. These chemotaxonomic data indicated that strain DQS3-9A1(T) differs from the present members of the suborder Corynebacterineae. Therefore, the creation of Amycolicicoccus subflavus gen. nov., sp. nov. is proposed, with DQS3-9A1(T) (=DSM 45089(T)=CGMCC 4.3532(T)) as the type strain.
两株新的放线菌，命名为 DQS3-9A1(T) 和 DQS3-9A2，从中国胜利油田受原油污染的盐土中分离得到。基于 16S rRNA 基因序列分析，这两株菌与分枝杆菌属最为密切相关（相似度为 92.7-94.9%），并在棒状杆菌亚目中形成一个独特的分支。此外，细胞壁中的主要糖阿拉伯糖和半乳糖支持菌株 DQS3-9A1(T) 与分枝杆菌科成员的归属。然而，菌株 DQS3-9A1(T) 不含分枝菌酸，并且两种分离株的主要menaquinone 均为 MK-8（85.5%）。菌株 DQS3-9A1(T) 的主要细胞脂肪酸为 C(16:0)（20.5%）、10-甲基 C(17:0)（19.3%）、10-甲基 C(18:0)（16.1%）、总和特征 3（11.4%）、C(15:0)（11.3%）、C(17:0)（5.0%）和 C(17:1)omega8c（5.0%）。菌株 DQS3-9A1(T) 的极性脂由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、磷脂酰肌醇和一种未知的含葡萄糖胺的磷脂组成。这些化学分类学数据表明，菌株 DQS3-9A1(T) 与棒状杆菌亚目的现有成员不同。因此，建议创建 Amycolicicoccus subflavus 属，新种，DQS3-9A1(T)（=DSM 45089(T)=CGMCC 4.3532(T)）作为模式菌株。
