Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada; Institute of Medical Science, University of Toronto, Toronto, Ontario M5T 3H7, Canada; Department of Obstetrics and Gynaecology, University of Toronto, Toronto, Ontario, Canada.
Curr Opin Genet Dev. 2013 Oct;23(5):534-9. doi: 10.1016/j.gde.2013.07.004. Epub 2013 Aug 19.
The groundbreaking discovery of the induced pluripotent stem cells in 2006 brought a major paradigm shift in our understanding of the plasticity of differentiated cells. An unprecedented effort has been invested to elucidate the underpinning molecular mechanisms of the dramatic changes in cell properties, using the powerful secondary reprogramming systems (2°RS). This review outlines the key components of the 2°RS, its recent development and applications that have led to a deeper understanding of the 'magic' of induced cell fate changes.
2006 年诱导多能干细胞的开创性发现，使我们对分化细胞的可塑性的认识发生了重大转变。人们投入了前所未有的努力，利用强大的次级重编程系统（2°RS），阐明细胞特性剧烈变化的潜在分子机制。本综述概述了 2°RS 的关键组成部分、其最新发展和应用，这些研究加深了我们对诱导细胞命运变化的“魔力”的理解。
