Gene Regulation, Stem Cells and Cancer Program, Center for Genomic Regulation and Pompeu Fabra University, Barcelona, Spain.
Curr Opin Genet Dev. 2013 Oct;23(5):562-7. doi: 10.1016/j.gde.2013.07.003. Epub 2013 Aug 19.
The activity of transcription factors (TFs) has empowered the reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) and alternative lineages. Elucidation of the molecular mechanisms underpinning these processes has expanded our understanding of how novel gene expression programs become activated while old ones are silenced. How TFs modify chromatin and activate requisite enzymes during these processes has recently been discussed in several excellent reviews. Here we discuss the questions of how ectopically expressed TFs access chromatin to modulate enhancers and establish a novel transcriptome during cell reprogramming, lineage conversions and differentiation.
转录因子 (TFs) 的活性使分化细胞重编程为诱导多能干细胞 (iPSCs) 和替代谱系成为可能。阐明这些过程的分子机制扩展了我们对新基因表达程序如何被激活而旧程序被沉默的理解。TFs 在这些过程中如何修饰染色质并激活必需的酶，最近在一些优秀的综述中进行了讨论。在这里，我们讨论了异位表达的 TFs 如何访问染色质来调节增强子并在细胞重编程、谱系转化和分化过程中建立新的转录组的问题。
