Anthony C
Biochem J. 1975 Feb;146(2):289-98. doi: 10.1042/bj1460289.
Pseudomonas AM1 contains cytochromes a, b and c and more than one CO-binding pigment (cytochrome a3, cytochrome c and possibly a cytochrome o). The soluble cytochrome c has been purified; its isoelectric point is low and its molecular weight is 20000. This cytochrome is reduced in whole bacteria by all oxidizable substrates at rates determined by the primary dehydrogenases. A mutant lacking cytochrome c oxidizes all substrates except methanol, ethanol and methylamine; these no longer support growth. The role of cytochrome c in electron transport in Pseudomonas AM1 is discussed.
假单胞菌AM1含有细胞色素a、b和c以及不止一种与一氧化碳结合的色素(细胞色素a3、细胞色素c,可能还有细胞色素o)。可溶性细胞色素c已被纯化;其等电点较低,分子量为20000。这种细胞色素在完整细菌中可被所有可氧化底物还原,还原速率由初级脱氢酶决定。一种缺乏细胞色素c的突变体能够氧化除甲醇、乙醇和甲胺以外的所有底物;而这些底物不再支持其生长。本文讨论了细胞色素c在假单胞菌AM1电子传递中的作用。