O'Keeffe D T, Anthony C
Biochem J. 1980 Aug 15;190(2):481-4. doi: 10.1042/bj1900481.
Cytochromes cH and cL were autoreduced at high pH (pK greater than 10) and the autoreduced cytochromes reacted with CO. The autoreduction was first-order with respect to oxidized cytochrome c and was reversible by lowering the pH. Pure methanol dehydrogenase reduced cytochrome c (in the absence of methanol) by lowering the pK for autoreduction to less than 8.5. A mechanism is proposed for the autoreduction of cytochrome c and its involvement in the reaction with methanol dehydrogenase.
细胞色素cH和cL在高pH值(pK大于10)时会自动还原,且自动还原的细胞色素会与CO反应。自动还原对氧化型细胞色素c呈一级反应,通过降低pH值可使其逆转。纯甲醇脱氢酶通过将自动还原的pK降低至小于8.5,从而在(无甲醇的情况下)还原细胞色素c。本文提出了一种细胞色素c自动还原的机制及其在与甲醇脱氢酶反应中的作用。