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兼性甲基营养菌假单胞菌AM1的甲醇脱氢酶与自身可还原细胞色素c之间的相互作用

The interaction between methanol dehydrogenase and the autoreducible cytochromes c of the facultative methylotroph Pseudomonas AM1.

作者信息

O'Keeffe D T, Anthony C

出版信息

Biochem J. 1980 Aug 15;190(2):481-4. doi: 10.1042/bj1900481.

Abstract

Cytochromes cH and cL were autoreduced at high pH (pK greater than 10) and the autoreduced cytochromes reacted with CO. The autoreduction was first-order with respect to oxidized cytochrome c and was reversible by lowering the pH. Pure methanol dehydrogenase reduced cytochrome c (in the absence of methanol) by lowering the pK for autoreduction to less than 8.5. A mechanism is proposed for the autoreduction of cytochrome c and its involvement in the reaction with methanol dehydrogenase.

摘要

细胞色素cH和cL在高pH值(pK大于10)时会自动还原,且自动还原的细胞色素会与CO反应。自动还原对氧化型细胞色素c呈一级反应,通过降低pH值可使其逆转。纯甲醇脱氢酶通过将自动还原的pK降低至小于8.5,从而在(无甲醇的情况下)还原细胞色素c。本文提出了一种细胞色素c自动还原的机制及其在与甲醇脱氢酶反应中的作用。

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引用本文的文献

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Methanol dehydrogenase: mechanism of action.甲醇脱氢酶:作用机制
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本文引用的文献

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Spectroscopic investigation of rubredoxin and ferredoxin.
Nature. 1966 Nov 5;212(5062):590-3. doi: 10.1038/212590a0.
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A novel coenzyme from bacterial primary alcohol dehydrogenases.
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