盾叶薯蓣的花中的甾体皂苷。

Steroidal saponins from the flowers of Dioscorea bulbifera var. sativa.

机构信息

Department of Chemistry, Faculty of Science, University of Dschang, Box 183, Dschang, Cameroon; Institut für Pharmazie (Pharmazeutische Biologie), Freie Universität Berlin, Königin-Luise-Str. 2-4, D-14195 Berlin, Germany.

出版信息

Phytochemistry. 2013 Nov;95:341-50. doi: 10.1016/j.phytochem.2013.07.020. Epub 2013 Aug 19.

DOI:10.1016/j.phytochem.2013.07.020

PMID:23969106

Abstract

Eleven steroidal saponins, dioscoreanosides A-K, along with five known congeners, were isolated from the flowers of Dioscorea bulbifera var. sativa. Their structures were established by extensive NMR experiments in conjunction with mass spectrometry. The isolated compounds were tested for cytotoxicity against urinary bladder carcinoma cells (ECV-304 cells). Our results revealed a moderate activity for spiroconazol A (15), pennogenin 3-O-α-l-rhamnopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→4)-[α-l-rhamnopyranosyl-(1→2)]-β-d-glucopyranoside (12), and 26-O-ß-d-glucopyranosyl-(25R)-5-en-furost-3ß,17α,22α,26-tetraol-3-O-α-l-rhamnopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→4)-[α-l-rhamnopyranosyl-(1→2)]-β-d-glucopyranoside (13).

摘要

从盾叶薯蓣的花中分离得到 11 种甾体皂甙，薯蓣皂甙 A-K，以及 5 种已知同系物。通过广泛的 NMR 实验结合质谱确定了它们的结构。对分离得到的化合物进行了细胞毒性测试，以评估它们对膀胱癌细胞（ECV-304 细胞）的作用。结果表明，螺旋甾酮 A（15）、绵马贯众酮 3-O-α-l-鼠李吡喃糖基-(1→4)-α-l-鼠李吡喃糖基-(1→4)-[α-l-鼠李吡喃糖基-(1→2)]-β-d-吡喃葡萄糖苷（12）和 26-O-β-d-吡喃葡萄糖基-(25R)-5-烯-呋甾-3β,17α,22α,26-四醇-3-O-α-l-鼠李吡喃糖基-(1→4)-α-l-鼠李吡喃糖基-(1→4)-[α-l-鼠李吡喃糖基-(1→2)]-β-d-吡喃葡萄糖苷（13）具有中等的细胞毒性。