Tabopda Turibio Kuiate, Mitaine-Offer Anne-Claire, Tanaka Chiaki, Miyamoto Tomofumi, Mirjolet Jean-François, Duchamp Olivier, Ngadjui Bonaventure Tchaleu, Lacaille-Dubois Marie-Aleth
EA 4267 (FDE/UFC), Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Bourgogne, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon CEDEX, France; Département de Chimie Organique, Université de Yaoundé 1, BP 812, Yaoundé, Cameroun.
EA 4267 (FDE/UFC), Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Bourgogne, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon CEDEX, France.
Fitoterapia. 2014 Sep;97:198-203. doi: 10.1016/j.fitote.2014.06.006. Epub 2014 Jun 11.
Three new steroidal saponins, named diospreussinosides A-C (1-3), along with two known ones (4, 5) were isolated from rhizomes of Dioscorea preussii. Their structures were elucidated mainly by 1D and 2D NMR spectroscopic analysis and mass spectrometry as (25S)-17α,25-dihydroxyspirost-5-en-3β-yl-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside (1), (25S)-17α,25-dihydroxyspirost-5-en-3β-yl-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (2), and (24S,25R)-17α,24,25-trihydroxyspirost-5-en-3β-yl-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (3). The spirostane-type skeleton of compound 3 possessing an unusual dihydroxylation pattern on the F-ring is reported for the first time. Cytotoxicity of compounds 2-5 was evaluated against two human colon carcinoma cell lines (HT-29 and HCT 116).
从薯蓣属植物普氏薯蓣的根茎中分离出三种新的甾体皂苷，命名为薯蓣普氏皂苷A - C（1 - 3），以及两种已知的甾体皂苷（4, 5）。它们的结构主要通过一维和二维核磁共振光谱分析以及质谱法确定为（25S）-17α,25 - 二羟基螺甾 - 5 - 烯 - 3β - 基 - O - α - L - 鼠李吡喃糖基 -（1→4）- α - L - 鼠李吡喃糖基 -（1→4）- β - D - 葡萄糖吡喃糖苷（1）、（25S）-17α,25 - 二羟基螺甾 - 5 - 烯 - 3β - 基 - O - α - L - 鼠李吡喃糖基 -（1→4）- α - L - 鼠李吡喃糖基 -（1→4）-[α - L - 鼠李吡喃糖基 -（1→2）]- β - D - 葡萄糖吡喃糖苷（2）和（24S,25R）-17α,24,25 - 三羟基螺甾 - 5 - 烯 - 3β - 基 - O - α - L - 鼠李吡喃糖基 -（1→4）- α - L - 鼠李吡喃糖基 -（1→4）-[α - L - 鼠李吡喃糖基 -（1→2）]- β - D - 葡萄糖吡喃糖苷（3）。首次报道了化合物3的螺旋甾烷型骨架在F环上具有不寻常的二羟基化模式。评估了化合物2 - 5对两种人结肠癌细胞系（HT - 29和HCT 116）的细胞毒性。
