Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, NL-6525 AJ Nijmegen, The Netherlands.
Org Biomol Chem. 2013 Oct 14;11(38):6439-55. doi: 10.1039/c3ob41329b. Epub 2013 Aug 23.
This review provides a literature overview of bioorthogonal ligation methods for protein modification, which have largely evolved over the last 15 years. Since 1990, various new reactions have been developed that do not involve naturally occurring functional handles. Especially the development of such so-called bioorthogonal ligations has significantly contributed to our ability to selectively modify biomolecules not only in the test tube, but also in living systems.
本文综述了近年来用于蛋白质修饰的生物正交连接方法，这些方法在过去 15 年中得到了很大的发展。自 1990 年以来，已经开发出了各种不涉及天然存在的功能基团的新反应。特别是这些所谓的生物正交连接的发展，极大地提高了我们不仅在试管中，而且在活体系统中选择性修饰生物分子的能力。
