Chen Xi, Wu Yao-Wen
Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany.
Org Biomol Chem. 2016 Jun 28;14(24):5417-39. doi: 10.1039/c6ob00126b. Epub 2016 Mar 4.
Over the years, there have been remarkable efforts in the development of selective protein labeling strategies. In this review, we deliver a comprehensive overview of the currently available bioorthogonal and chemoselective reactions. The ability to introduce bioorthogonal handles to proteins is essential to carry out bioorthogonal reactions for protein labeling in living systems. We therefore summarize the techniques that allow for site-specific "installation" of bioorthogonal handles into proteins. We also highlight the biological applications that have been achieved by selective chemical labeling of proteins.
多年来，在选择性蛋白质标记策略的开发方面付出了巨大努力。在本综述中，我们全面概述了目前可用的生物正交和化学选择性反应。将生物正交手柄引入蛋白质的能力对于在生物系统中进行蛋白质标记的生物正交反应至关重要。因此，我们总结了能够将生物正交手柄位点特异性“安装”到蛋白质中的技术。我们还强调了通过蛋白质的选择性化学标记所实现的生物学应用。
