Agra Isabela K R, Pires Luana L S, Carvalho Paulo S M, Silva-Filho Eurípedes A, Smaniotto Salete, Barreto Emiliano
Laboratório de Biologia Celular, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Campus A.C. Simões, 57072-970 Maceió Al, Brasil.
An Acad Bras Cienc. 2013 Sep;85(3):945-54. doi: 10.1590/S0001-37652013005000049.
The decoction of the stem barks from Bowdichia virgilioides KUNTH is a folk remedy used to treat inflammatory disorders in Latin American and Brazil. In the present study, the wound healing activity of aqueous extract of the stem bark from B. virgilioides, called AEBv, was evaluated by the rate of healing by wound contraction and period of epithelization at different days post-wound using the wound excisional model. On day 9, the AEBv-treated animals exhibited significative reduction in the wound area when compared with controls. In wound infected with S. aureus, the AEBv significantly improved the wound contraction when compared to the saline-treated mice. The histological analysis showed that AEBv induced a collagen deposition, increase in the fibroblast count and few inflammatory cells than compared to saline-treated group. The expression of collagen type I was increased in the group treated with AEBv as indicated by immunohistochemical staining. In vitro, the AEBv was effective only against S. aureus but not against P. aeruginosa. Together, the results of this study demonstrate, for the first time, the healing and antimicrobiological effects of aqueous extract of the stem bark from B. virgilioides in the therapy of skin wounds.
Virgilioides KUNTH茎皮的煎剂是拉丁美洲和巴西用于治疗炎症性疾病的民间疗法。在本研究中，使用伤口切除模型，通过伤口收缩的愈合率和伤口后不同天数的上皮形成期，评估了 Virgilioides 茎皮水提取物（称为AEBv）的伤口愈合活性。在第9天，与对照组相比，接受AEBv治疗的动物伤口面积显著减小。在感染金黄色葡萄球菌的伤口中，与盐水处理的小鼠相比，AEBv显著改善了伤口收缩。组织学分析表明，与盐水处理组相比，AEBv诱导了胶原蛋白沉积、成纤维细胞数量增加且炎症细胞较少。免疫组织化学染色显示，用AEBv处理的组中I型胶原蛋白的表达增加。在体外，AEBv仅对金黄色葡萄球菌有效，而对铜绿假单胞菌无效。总之，本研究结果首次证明了 Virgilioides 茎皮水提取物在皮肤伤口治疗中的愈合和抗菌作用。
