羽扇醇可改善成纤维细胞和内皮细胞的功能，加速小鼠皮肤伤口愈合。
Uvaol Improves the Functioning of Fibroblasts and Endothelial Cells and Accelerates the Healing of Cutaneous Wounds in Mice.
机构信息
Laboratory of Cell Biology, Federal University of Alagoas, 57072-900 Maceió, Brazil.
Garanhuns College of Science, Education and Technology, University of Pernambuco, 55294-902 Garanhuns, Brazil.
出版信息
Molecules. 2020 Oct 28;25(21):4982. doi: 10.3390/molecules25214982.
Uvaol is a natural pentacyclic triterpene that is widely found in olives and virgin olive oil, exerting various pharmacological properties. However, information remains limited about how it affects fibroblasts and endothelial cells in events associated with wound healing. Here, we report the effect of uvaol in the in vitro and in vivo healing process. We show the positive effects of uvaol on migration of fibroblasts and endothelial cells in the scratch assay. Protein synthesis of fibronectin and laminin (but not collagen type I) was improved in uvaol-treated fibroblasts. In comparison, tube formation by endothelial cells was enhanced after uvaol treatment. Mechanistically, the effects of uvaol on cell migration involved the PKA and p38-MAPK signaling pathway in endothelial cells but not in fibroblasts. Thus, the uvaol-induced migratory response was dependent on the PKA pathway. Finally, topical treatment with uvaol caused wounds to close faster than in the control treatment using experimental cutaneous wounds model in mice. In conclusion, uvaol positively affects the behavior of fibroblasts and endothelial cells, potentially promoting cutaneous healing.
乌索酸是一种天然五环三萜，广泛存在于橄榄和特级初榨橄榄油中，具有多种药理作用。然而，关于它如何影响创伤愈合相关事件中的成纤维细胞和内皮细胞的信息仍然有限。在这里，我们报告了乌索酸在体外和体内愈合过程中的作用。我们显示了乌索酸对划痕试验中成纤维细胞和内皮细胞迁移的积极影响。乌索酸处理的成纤维细胞中纤连蛋白和层粘连蛋白（但不是 I 型胶原）的蛋白合成得到改善。相比之下，内皮细胞的管形成在乌索酸处理后得到增强。从机制上讲，乌索酸对细胞迁移的影响涉及内皮细胞中的 PKA 和 p38-MAPK 信号通路，但在成纤维细胞中则没有。因此，乌索酸诱导的迁移反应依赖于 PKA 通路。最后，在使用小鼠皮肤创伤模型的实验中，与对照组相比，局部应用乌索酸可使创面更快愈合。总之，乌索酸可积极影响成纤维细胞和内皮细胞的行为，可能促进皮肤愈合。
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