An Yan, Li Jianing, Liu Yajun, Fan Mingxing
Department Of Spine Surgery, Beijing Jishuitan Hospital, Beijing 100035, China.
Beijing Jishuitan Hospital Search for articles by this author in MEDLINE®, Beijing, China.
Transl Neurosci. 2020 May 29;11(1):182-191. doi: 10.1515/tnsci-2020-0106. eCollection 2020.
The present study was performed to evaluate the effects of ormosanine against spinal cord injury (SCI) in rats and to examine the possible molecular mechanism of action. SCI was induced using an impactor device, and rats were treated with ormosanine 10, 50 or 100 mg/kg, p.o., for 10 days after induction of SCI. The effect of ormosanine on SCI was determined by estimating neurological functions and cytokines and parameters of oxidative stress level were estimated in SCI rats. Quantitative reverse transcription polymerase chain reaction, Western blotting analysis and histopathological study were performed on spinal tissue of SCI rats. The data suggested that treatment with ormosanine reversed the alterations of neurological function in SCI rats. Moreover, the levels of cytokines, oxidative stress and reactive oxygen species production were reduced in the ormosanine treatment group compared to the SCI group. The levels of calpain and neuronal nitric oxide synthase activity were significantly reduced in the spinal tissue of the ormosanine treatment group compared to the SCI group. Moreover, ormosanine treatment reduced the percentage of viable neurons in the spinal tissue of SCI rats. In conclusion, the results of this study showed that ormosanine treatment had a protective effect against neuronal injury in spinal cord-injured rats by regulating the peroxynitrite/calpain activity.
本研究旨在评估牛心朴子碱对大鼠脊髓损伤（SCI）的影响，并探讨其可能的分子作用机制。使用撞击装置诱导大鼠脊髓损伤，在脊髓损伤诱导后，大鼠分别接受10、50或100mg/kg牛心朴子碱灌胃治疗，持续10天。通过评估神经功能来确定牛心朴子碱对脊髓损伤的作用，并对脊髓损伤大鼠的细胞因子和氧化应激水平参数进行评估。对脊髓损伤大鼠的脊髓组织进行定量逆转录聚合酶链反应、蛋白质免疫印迹分析和组织病理学研究。数据表明，牛心朴子碱治疗可逆转脊髓损伤大鼠神经功能的改变。此外，与脊髓损伤组相比，牛心朴子碱治疗组的细胞因子水平、氧化应激和活性氧生成均降低。与脊髓损伤组相比，牛心朴子碱治疗组脊髓组织中钙蛋白酶和神经元型一氧化氮合酶活性水平显著降低。此外，牛心朴子碱治疗降低了脊髓损伤大鼠脊髓组织中存活神经元的百分比。总之，本研究结果表明，牛心朴子碱治疗通过调节过氧亚硝酸盐/钙蛋白酶活性对脊髓损伤大鼠的神经元损伤具有保护作用。
