da Silva Robson Alves, Dihl Rafael Rodrigues, Nascimento e Santos Débora, de Abreu Bianca Regina Ribas, de Lima Alessandro, de Andrade Heloisa Helena Rodrigues, Lehmann Mauricio
Laboratório de Toxicidade Genética (TOXIGEN), Programa de Pós-Graduação em Genética e Toxicologia Aplicada e Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada à Saúde, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil; Instituto Federal de Educação, Ciência e Tecnologia do Piauí (IFPI), Teresina, PI, Brazil.
Food Chem Toxicol. 2013 Dec;62:61-7. doi: 10.1016/j.fct.2013.08.026. Epub 2013 Aug 20.
In vitro chemical properties and antioxidant potential and in vivo mutagenic activity of honey-sweetened cashew apple nectar (HSCAN), a beverage produced from the cashew pseudo-fruit (Anacardium occidentale L.) and of its constituents were assessed. Analytical procedures were carried out to investigate the honey used in the HSCAN preparation, and the results observed are in accordance with Brazilian legal regulations, except for diastase number. HSCAN and pulp were investigated for ascorbic acid, carotenoid, anthocyanin and total phenolic contents, and both showed high acid ascorbic concentrations. Antioxidant capacity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and/or β-carotene/linoleic acid systems were applied and demonstrated a weak antioxidant capacity of honey and HSCAN, but cashew apple pulp demonstrated high antioxidant capacity. A weakly positive mutagenic effect of cashew pulp 20% was observed using the somatic mutation and recombination test (SMART) in Drosophila melanogaster only in the high-bioactivation (HB) cross. On the contrary, HSCAN was not mutagenic in both standard and high bioactivation crosses. HSCAN exhibited slight antioxidant activity, which could be associated with the high amount of ascorbic acid found in the samples evaluated. The beverage prepared did not induce DNA damage in somatic cells of D. melanogaster, which means that it is neither mutagenic nor recombinagenic in this test system.
对用蜂蜜调味的腰果苹果花蜜（HSCAN）及其成分进行了体外化学性质、抗氧化潜力和体内诱变活性评估。HSCAN是一种由腰果假果（腰果，Anacardium occidentale L.）制成的饮料。对制备HSCAN所用的蜂蜜进行了分析程序，除淀粉酶值外，观察结果符合巴西法律法规。对HSCAN和果肉的抗坏血酸、类胡萝卜素、花青素和总酚含量进行了研究，两者均显示出高浓度的抗坏血酸。使用2,2-二苯基-1-苦基肼自由基（DPPH）和/或β-胡萝卜素/亚油酸系统测定抗氧化能力，结果表明蜂蜜和HSCAN的抗氧化能力较弱，但腰果苹果果肉具有较高的抗氧化能力。仅在高生物活化（HB）杂交实验中，利用果蝇的体细胞突变和重组试验（SMART）观察到20%腰果果肉有微弱的诱变作用。相反，在标准和高生物活化杂交实验中，HSCAN均无诱变作用。HSCAN表现出轻微的抗氧化活性，这可能与所评估样品中发现的大量抗坏血酸有关。所制备的饮料不会诱导黑腹果蝇体细胞中的DNA损伤，这意味着在该测试系统中它既不具有诱变性也不具有重组性。
