Vale Nuno, Gouveia Maria João, Botelho Mónica, Sripa Banchob, Suttiprapa Sutas, Rinaldi Gabriel, Gomes Paula, Brindley Paul J, Correia da Costa José Manuel
CIQUP, Chemistry and Biochemistry Department, Faculty of Sciences, University of Porto, Rua Campo Alegre, 687, 4169-007 Porto, Portugal.
Parasitol Int. 2013 Dec;62(6):535-42. doi: 10.1016/j.parint.2013.08.001. Epub 2013 Aug 20.
Liquid chromatography in tandem mass spectrometry (LC-MS/MS) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. Here, we used LC-MS/MS to investigate in soluble extracts of the adult developmental stage of Opisthorchis viverrini from experimentally infected hamsters. Using comparisons with known bile acids and the metabolites of estrogens, the LC-MS data indicated the existence of novel oxysterol derivatives in O. viverrini. Most of these derivatives were ramified at C-17, in similar fashion to bile acids and their conjugated salts. Several were compatible with the presence of an estrogen core, and/or hydroxylation of the steroid aromatic ring A, hydroxylation of both C-2 and C-3 of the steroid ring and further oxidation into an estradiol-2,3-quinone.
液相色谱串联质谱法（LC-MS/MS）已成为一种用于研究蠕虫寄生虫相关癌症中氧化甾醇（胆固醇的氧化衍生物）的信息丰富的工具。在此，我们使用LC-MS/MS对来自实验感染仓鼠的华支睾吸虫成虫发育阶段的可溶性提取物进行研究。通过与已知胆汁酸和雌激素代谢物进行比较，LC-MS数据表明华支睾吸虫中存在新型氧化甾醇衍生物。这些衍生物大多在C-17处有分支，其方式与胆汁酸及其共轭盐相似。有几种与雌激素核心的存在、和/或甾体芳香环A的羟基化、甾体环C-2和C-3的羟基化以及进一步氧化为雌二醇-2,3-醌相符合。
