State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Colloids Surf B Biointerfaces. 2013 Dec 1;112:146-54. doi: 10.1016/j.colsurfb.2013.07.048. Epub 2013 Aug 3.
As a biostable elastomer, the hydrophobicity of styrenic block copolymer (SBC) intensely limits its biomedical applications. In order to overcome such shortcoming, the SBC films were grafted with hyaluronic acid (HA) using a coupling agent. The surface chemistry of the modified films was examined by ATR-FTIR and XPS techniques, and the surface morphology was visually described by AFM. The biological performances of the HA-modified films were evaluated by a series of experiments, such as protein adsorption, platelet adhesion, and in vitro cytocompatibility. It was found that the HA-modified samples showed a low adhesiveness to fibroblast at the initial stage; however, it stimulated the growth of fibroblast. The L929 fibroblast growth presented a strong dependence on the molecular weight (MW) of HA. The samples modified with 17kDa HA exhibited the worst wettability and platelet adhesion, while providing the best results of supporting fibroblast proliferation.
作为一种生物稳定弹性体，苯乙烯嵌段共聚物（SBC）的疏水性极大地限制了其在生物医学中的应用。为了克服这一缺点，使用偶联剂将 SBC 薄膜接枝上透明质酸（HA）。通过 ATR-FTIR 和 XPS 技术对改性膜的表面化学性质进行了研究，通过 AFM 对表面形貌进行了直观描述。通过一系列实验，如蛋白质吸附、血小板黏附以及体外细胞相容性评估了 HA 改性膜的生物学性能。结果表明，HA 改性样品在初始阶段对成纤维细胞的黏附性较低；然而，它刺激了成纤维细胞的生长。L929 成纤维细胞的生长强烈依赖于 HA 的分子量（MW）。用 17kDa HA 改性的样品表现出最差的润湿性和血小板黏附性，同时为支持成纤维细胞增殖提供了最佳的效果。
