Department of Chemistry, McMaster University, 1280 Main St. W., Hamilton ON L8S 4M1, Canada.
Biomaterials. 2010 May;31(13):3471-8. doi: 10.1016/j.biomaterials.2010.01.069. Epub 2010 Feb 6.
Although silicones possess many useful properties as biomaterials, their hydrophobicity can be problematic. To a degree, this issue can be addressed by surface modification with hydrophilic polymers such as poly(ethylene glycol), but the resulting structures are usually not conducive to cell growth. In the present work, we describe the synthesis and characterization of covalently linked hyaluronic acid (HA) (35 kDa) to poly(dimethylsiloxane) (PDMS) elastomer surfaces. HA is of interest because of its known biological properties; its presence on a surface was expected to improve the biocompatibility of silicone materials for a wide range of bioapplications. HA was introduced with a coupling agent in two steps from high-density, tosyl-modified, poly(ethylene glycol) tethered silicone surfaces. All materials synthesized were characterized by water contact angle, ATR-FTIR, XPS and (13)C solid state NMR spectroscopy. Biological interactions with these modified silicone surfaces were assessed by examining interactions with fibrinogen as a model protein as well as determining the in vitro response of fibroblast (3T3) and human corneal epithelial cells relative to unmodified poly(dimethylsiloxane) controls. The results suggest that HA modification significantly enhances cell interactions while decreasing protein adsorption and may therefore be effective for improving biocompatibility of PDMS and other materials.
尽管硅酮作为生物材料具有许多有用的特性，但它们的疏水性可能是一个问题。在一定程度上，这个问题可以通过用亲水性聚合物（如聚乙二醇）对表面进行改性来解决，但得到的结构通常不利于细胞生长。在本工作中，我们描述了通过共价键将透明质酸（HA）（35 kDa）连接到聚二甲基硅氧烷（PDMS）弹性体表面的合成和表征。HA 之所以受到关注，是因为其具有已知的生物学特性；预计其存在于表面上会提高硅酮材料在广泛的生物应用中的生物相容性。HA 是通过两步从高密度、对甲苯磺酰基改性的、接枝有聚乙二醇的硅氧烷表面用偶联剂引入的。所有合成的材料都通过水接触角、ATR-FTIR、XPS 和（13）C 固态 NMR 光谱进行了表征。通过研究与纤维蛋白原的相互作用（作为模型蛋白）以及确定纤维母细胞（3T3）和人角膜上皮细胞相对于未改性 PDMS 的体外反应，评估了这些改性硅酮表面的生物学相互作用。结果表明，HA 改性显著增强了细胞相互作用，同时减少了蛋白质吸附，因此可能有效提高 PDMS 和其他材料的生物相容性。
