Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
Environ Pollut. 2013 Nov;182:385-91. doi: 10.1016/j.envpol.2013.07.039. Epub 2013 Aug 23.
A highly selective sample cleanup procedure combined with molecularly imprinted solid-phase extraction (MISPE) was developed for the isolation of gonyautoxins 2,3 (GTX2,3) from Alexandrium tamarense sample. The molecularly imprinted polymer microspheres (MIPMs) were prepared by suspension polymerization using caffeine as the dummy template molecule, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linker and polyvinyl alcohol as the dispersive reagent. The polymer microspheres were used as a selective sorbent for the solid-phase extraction of gonyautoxins 2,3. An off-line MISPE method followed by high-performance liquid chromatography (HPLC) with fluorescence detection for the analysis of gonyautoxins 2,3 was established. Finally, the extract samples from Alexandrium tamarense were analyzed. The results showed the imprinted polymer microspheres exhibited high affinity and selectivity for gonyautoxins 2,3. The interference matrix in the extract were obviously cleaned by MISPE and the extraction efficiency of gonyautoxins 2,3 in the sample ranged from 81.74% to 85.86%.
一种高选择性的样品净化程序与分子印迹固相萃取（MISPE）相结合，用于从亚历山大藻样品中分离 GTX2,3。采用悬浮聚合法，以咖啡因作为虚拟模板分子，甲基丙烯酸作为功能单体，乙二醇二甲基丙烯酸酯作为交联剂，聚乙烯醇作为分散剂，制备了分子印迹聚合物微球（MIPMs）。聚合物微球作为 GTX2,3 的固相萃取的选择性吸附剂。建立了 HPLC 荧光检测分析 GTX2,3 的离线 MISPE 方法。最后，对亚历山大藻提取物样品进行了分析。结果表明，印迹聚合物微球对 GTX2,3 具有高亲和力和选择性。MISPE 明显清除了提取物中的干扰基质，GTX2,3 在样品中的提取效率为 81.74%至 85.86%。
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