Lian Ziru, Wang Jiangtao
Marine College, Shandong University, Weihai 264209, China.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100,China.
Mar Pollut Bull. 2017 Sep 15;122(1-2):500-504. doi: 10.1016/j.marpolbul.2017.06.066. Epub 2017 Jun 26.
Gonyautoxins 1,4 (GTX1,4) from Alexandrium minutum samples were isolated selectively and recognized specifically by an innovative and effective extraction procedure based on molecular imprinting technology. Novel molecularly imprinted polymer microspheres (MIPMs) were prepared by double-templated imprinting strategy using caffeine and pentoxifylline as dummy templates. The synthesized polymers displayed good affinity to GTX1,4 and were applied as sorbents. Further, an off-line molecularly imprinted solid-phase extraction (MISPE) protocol was optimized and an effective approach based on the MISPE coupled with HPLC-FLD was developed for selective isolation of GTX1,4 from the cultured A. minutum samples. The separation method showed good extraction efficiency (73.2-81.5%) for GTX1,4 and efficient removal of interferences matrices was also achieved after the MISPE process for the microalgal samples. The outcome demonstrated the superiority and great potential of the MISPE procedure for direct separation of GTX1,4 from marine microalgal extracts.
采用基于分子印迹技术的创新有效提取方法，从微小亚历山大藻样本中选择性分离并特异性识别了膝沟藻毒素1,4（GTX1,4）。以咖啡因和己酮可可碱作为虚拟模板，通过双模板印迹策略制备了新型分子印迹聚合物微球（MIPMs）。合成的聚合物对GTX1,4表现出良好的亲和力，并用作吸附剂。此外，优化了离线分子印迹固相萃取（MISPE）方案，开发了一种基于MISPE与HPLC-FLD联用的有效方法，用于从培养的微小亚历山大藻样本中选择性分离GTX1,4。该分离方法对GTX1,4具有良好的提取效率（73.2-81.5%），并且在对微藻样本进行MISPE处理后，也有效去除了干扰基质。结果证明了MISPE方法在直接从海洋微藻提取物中分离GTX1,4方面的优越性和巨大潜力。
