Fabisiak Michał, Podgórska Katarzyna, Skrzypiec Ewelina, Szczotka Anna, Stadejek Tomasz
Warsaw University of Life Sciences - SGGW Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine Nowoursynowska 159c 02-776 Warsaw Poland.
Acta Vet Hung. 2013 Dec;61(4):529-36. doi: 10.1556/AVet.2013.027.
PCV2 and PRRSV are two important pathogens of domestic swine. There is considerable evidence that the infection is also present in wild boars. Meat juice provides an alternative to serum for antibody testing, and it has been used in testing for many important porcine infectious diseases. Samples of brachial muscle were collected from 142 wild boars shot in different regions of Poland during the 2006/2007 and 2007/2008 hunting seasons. Meat juice harvested from muscle samples was tested using an ELISA test specific for PCV2 and PRRSV antibodies. Additionally, IgG and IgM antibodies specific for PCV2 were detected in order to estimate the status of the PCV2 infection. Only one of the tested meat juice samples was positive for PRRSV (0.7%), and 68 out of 142 (47.9%) samples were positive for PCV2. Of the positive animals, 4 (2.8%) had an antibody profile suggesting active infection, 2 (1.4%) early active infection, and 62 (43.7%) late infection. Also, a lack of association between the age of the animals and the presence of antibodies related to the infection was noticed.
猪圆环病毒2型（PCV2）和猪繁殖与呼吸综合征病毒（PRRSV）是家猪的两种重要病原体。有大量证据表明，野猪中也存在这种感染。肉汁为抗体检测提供了一种替代血清的方法，并且已用于多种重要猪传染病的检测。在2006/2007年和2007/2008年狩猎季节，从波兰不同地区射杀的142头野猪身上采集了肱肌样本。使用针对PCV2和PRRSV抗体的ELISA检测法对从肌肉样本中获取的肉汁进行检测。此外，还检测了针对PCV2的IgG和IgM抗体，以评估PCV2感染状况。检测的肉汁样本中只有1份PRRSV呈阳性（0.7%），142份样本中有68份（47.9%）PCV2呈阳性。在阳性动物中，4头（2.8%）的抗体谱表明为活跃感染，2头（1.4%）为早期活跃感染，62头（43.7%）为晚期感染。此外，还注意到动物年龄与感染相关抗体的存在之间没有关联。
