National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2 (Yeo, Yang, Nfon); Department of Animal Science, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, Manitoba R3T 2N2 (Yeo, Nyachoti, Nfon).
Can J Vet Res. 2022 Apr;86(2):147-152.
Established test methods for detecting foot-and-mouth disease virus (FMDV) rely on sample collection from live animals. However, circumstances exist in which it is not possible to collect the desired samples. Meat juice has been explored as an alternative for the detection of FMDV and has previously proven successful by real-time reverse transcription polymerase chain reaction and lateral flow strip test. Meat juice has not yet been assessed for the detection of antibodies to FMDV. This study, therefore, evaluated meat juice for the detection of antibodies to structural proteins by existing serotype-specific solid phase competitive enzyme-linked immunosorbent assays. Antibodies to FMDV structural proteins were detected in meat juice from experimentally infected pigs beginning 6- or 7-days post-infection (DPI) and continued until 21 to 28 DPI. Sera were tested in tandem and followed similar antibody detection patterns. The results show that meat juice can be used for detection of anti- FMDV structural protein antibodies.
已建立的用于检测口蹄疫病毒 (FMDV) 的测试方法依赖于从活体动物采集样本。然而，在某些情况下，无法采集到所需的样本。肉汁已被探索作为 FMDV 检测的替代物，并且之前已经通过实时逆转录聚合酶链反应和侧向流动条测试成功证明。肉汁尚未被评估用于检测针对 FMDV 的抗体。因此，本研究通过现有的血清型特异性固相竞争酶联免疫吸附试验评估了肉汁用于检测针对结构蛋白的抗体。从实验感染的猪开始，在感染后 6 或 7 天 (DPI) 时即可在肉汁中检测到针对 FMDV 结构蛋白的抗体，并持续到 21 至 28 DPI。血清依次进行测试，并呈现出相似的抗体检测模式。结果表明，肉汁可用于检测抗 FMDV 结构蛋白抗体。
