Hammer Ralf, Ritzmann Mathias, Palzer Andreas, Lang Christiane, Hammer Birgit, Pesch Stefan, Ladinig Andrea
Tierarztpraxis im alten Forstamt, Jahnstrasse 4, 72108 Rottenburg am Neckar, Germany.
J Wildl Dis. 2012 Jan;48(1):87-94. doi: 10.7589/0090-3558-48.1.87.
Samples were collected from 203 wild boars (Sus scrofa) hunted in Baden-Wurtemburg, Germany from November-January 2008 and 2009. Samples from the lung and tonsil were analyzed by quantitative polymerase chain reaction (qPCR) for porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European type) and type 2 (American type). A qPCR to detect porcine circovirus type 2 (PCV2)-specific genome was performed on tissue homogenates including lung, tonsils, and inguinal lymph nodes. Serum samples were tested for antibodies against PRRSV and PCV2 by enzyme-linked immunosorbent assay (ELISA). No PRRSV was detected in any of the 203 samples and one sample had detectable antibodies against PRRSV. We detected PCV2 in organ materials from 103 wild boars with a prevalence of 50.7%. The number of wild boars positive for PCV2 by PCR varied according to the population density of wild boars among woodlands. More positive samples were detected in woodlands with a high density of wild boars. We found no correlation between the number of PCV2-positive wild boars and the density of domestic pigs in the surrounding area. The number of wild boars positive for antibodies against PCV2 by the INGEZIM Circovirus IgG/IgM test kit was low (53 sera positive for IgG- and three sera positive for IgM-antibodies) in comparison to the higher positive results from the INGEZIM CIRCO IgG test kit (102 positive and 12 inconclusive results).
2008年11月至2009年1月期间，从德国巴登-符腾堡州捕猎的203头野猪（野猪）身上采集了样本。对来自肺和扁桃体的样本进行了定量聚合酶链反应（qPCR）分析，以检测1型（欧洲型）和2型（美国型）猪繁殖与呼吸综合征病毒（PRRSV）。对包括肺、扁桃体和腹股沟淋巴结在内的组织匀浆进行了检测猪圆环病毒2型（PCV2）特异性基因组的qPCR。通过酶联免疫吸附测定（ELISA）检测血清样本中针对PRRSV和PCV2的抗体。在203个样本中均未检测到PRRSV，有1个样本检测到针对PRRSV的抗体。我们在103头野猪的器官材料中检测到了PCV2，流行率为50.7%。通过PCR检测呈PCV2阳性的野猪数量因林地中野猪的种群密度而异。在野猪密度高的林地中检测到更多阳性样本。我们发现PCV2阳性野猪的数量与周边地区家猪的密度之间没有相关性。与INGEZIM CIRCO IgG检测试剂盒的较高阳性结果（102个阳性和12个不确定结果）相比，使用INGEZIM Circovirus IgG/IgM检测试剂盒检测到的针对PCV2抗体呈阳性的野猪数量较少（53份血清IgG呈阳性，3份血清IgM呈阳性）。
