School of Life Science and Technology, Xi'an Jiaotong University, Xianning Rd., Xi'an 710049, PR China.
Bioresour Technol. 2010 Jun;101(11):4029-33. doi: 10.1016/j.biortech.2010.01.042. Epub 2010 Feb 9.
A cellulolytic, hydrogen-producing bacterium (Clostridiumthermocellum DSM 1237) and a non-cellulolytic, hydrogen-producing bacterium (Clostridiumthermopalmarium DSM 5974) were co-cultured at 55 degrees C, using cellulose as the sole substrate. At a low load of cellulose (filter paper, 4.5g/L), yeast extract had a significant effect on cellulose degradation and hydrogen production. The extent of cellulose utilization and hydrogen production displayed a linear relationship with the logarithm of the yeast extract concentration, and the optimal weight ratio of yeast extract to cellulose was 1:1. At a high load of filter paper (9g/L), an alkali chemical was required to maintain efficient cellulose degradation. As the KHCO3 concentration increased from 0 to 60mM, the utilized cellulose increased from 1.23g/L (13.5%) to 8.59g/L (94.3%), and maximum hydrogen production (1387ml/L of culture) occurred at 40mM KHCO(3). Increasing the inoculation ratio of C. thermopalmarium to C. thermocellum from 0.05:1 to 0.17:1 had little influence on hydrogen production, probably because of the limited availability of soluble sugar in the medium during the early stages of the co-culture.
采用纤维素作为唯一底物，在 55°C 下对产纤维素、产氢菌（热纤梭菌 DSM 1237）和非产纤维素、产氢菌（嗜热解纤维梭菌 DSM 5974）进行共培养。在低纤维素负荷（滤纸，4.5g/L）下，酵母提取物对纤维素降解和产氢有显著影响。纤维素利用率和产氢量与酵母提取物浓度的对数呈线性关系，酵母提取物与纤维素的最佳重量比为 1:1。在高滤纸负荷（9g/L）下，需要碱化学物质来维持高效的纤维素降解。随着 KHCO3 浓度从 0 增加到 60mM，利用的纤维素从 1.23g/L（13.5%）增加到 8.59g/L（94.3%），在 40mM KHCO(3) 时最大产氢量（1387ml/L 培养物）发生。将嗜热解纤维梭菌与热纤梭菌的接种比例从 0.05:1 增加到 0.17:1 对产氢几乎没有影响，这可能是因为在共培养的早期阶段，培养基中可溶性糖的有限可用性。
