NSB Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California.
J Nucl Med. 2013 Oct;54(10):1820-4. doi: 10.2967/jnumed.112.118497. Epub 2013 Aug 26.
We report on a radiopharmaceutical imaging platform designed to capture the kinetics of cellular responses to drugs.
A portable in vitro molecular imaging system comprising a microchip and a β-particle imaging camera permitted routine cell-based radioassays of small numbers of either suspended or adherent cells. We investigated the kinetics of responses of model lymphoma and glioblastoma cancer cell lines to (18)F-FDG uptake after drug exposure. Those responses were correlated with kinetic changes in the cell cycle or with changes in receptor tyrosine kinase signaling.
The platform enabled direct radioassays of multiple cell types and yielded results comparable to those from conventional approaches; however, the platform used smaller sample sizes, permitted a higher level of quantitation, and did not require cell lysis.
The kinetic analysis enabled by the platform provided a rapid (≈ 1 h) drug screening assay.
我们报告了一种放射性药物成像平台，旨在捕捉细胞对药物反应的动力学。
一种由微芯片和β粒子成像相机组成的便携式体外分子成像系统，允许对少量悬浮或贴壁细胞进行常规基于细胞的放射分析。我们研究了模型淋巴瘤和神经胶质瘤癌细胞系在药物暴露后对（18）F-FDG 摄取的反应动力学。这些反应与细胞周期中的动力学变化或受体酪氨酸激酶信号的变化相关联。
该平台能够直接对多种细胞类型进行放射分析，结果与传统方法相当；然而，该平台使用的样本量更小，允许更高的定量水平，并且不需要细胞裂解。
该平台提供的动力学分析提供了一种快速（≈1 小时）的药物筛选测定。
