利用高温细菌联合体从厨房垃圾中生产光学纯 L-乳酸的新工艺。
A novel production process for optically pure L-lactic acid from kitchen refuse using a bacterial consortium at high temperatures.
机构信息
Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; Institute of Advanced Study, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
出版信息
Bioresour Technol. 2013 Oct;146:672-681. doi: 10.1016/j.biortech.2013.07.102. Epub 2013 Jul 29.
We investigated L-lactic acid production in static batch fermentation of kitchen refuse using a bacterial consortium from marine-animal-resource (MAR) composts at temperatures ranging from 30 to 65 °C. At relatively low temperatures butyric acid accumulated, whereas at higher temperatures L-lactic acid was produced. In particular, fermentation at 50 °C produced 34.5 g L(-1) L-lactic acid with 90% lactic acid selectivity and 100% optical purity. Denaturing gradient gel electrophoresis indicated that dominant bacteria present in the original MAR composts diminished rapidly and Bacillus coagulans strains became the dominant contributors to L-lactic acid production at 45, 50 and 55 °C. This is the first report of the achievement of 100% optical purity of L-lactic acid using a bacterial consortium.
我们研究了使用来自海洋动物资源（MAR）堆肥的细菌混合物在 30 至 65°C 的温度范围内进行静态分批发酵厨房垃圾中的 L-乳酸生产。在相对较低的温度下，丁酸积累，而在较高的温度下则产生 L-乳酸。特别是，在 50°C 下发酵可产生 34.5 g L(-1)L-乳酸，具有 90%的乳酸选择性和 100%的光学纯度。变性梯度凝胶电泳表明，原始 MAR 堆肥中存在的优势细菌迅速减少，凝结芽孢杆菌菌株成为在 45、50 和 55°C 下生产 L-乳酸的主要贡献者。这是使用细菌混合物实现 L-乳酸 100%光学纯度的首次报道。
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