SPF10 实时 PCR 与常规 PCR 联合 INNO-LiPA HPV Genotyping Extra 检测试剂盒在宫颈样本中人乳头瘤病毒检测和分型中的比较。
Comparison of SPF10 real-time PCR and conventional PCR in combination with the INNO-LiPA HPV Genotyping Extra assay for the detection and typing of human papillomavirus in cervical samples.
机构信息
Applied Molecular Biology Research (AMBIOR) Group, Laboratory of Cell Biology and Histology, University of Antwerp, Groenenborgerlaan 171, B2020 Antwerp, Belgium; Vaccine & Infectious Diseases Institute (VAXINFECTIO), University of Antwerp, Universiteitsplein 1, B2610 Antwerp, Belgium.
出版信息
J Virol Methods. 2013 Dec;194(1-2):113-7. doi: 10.1016/j.jviromet.2013.08.013. Epub 2013 Aug 23.
The novel SPF10 real-time PCR assay allows the simultaneous amplification and detection of the HPV target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. This study aims to evaluate the performance of the SPF10 real-time PCR in combination with the LiPA assay for HPV detection and typing in cervical samples. Thirty-nine cervical samples were subjected to the SPF10 conventional PCR in combination with the LiPA assay. Subsequently, the SPF10 real-time PCR was performed to enable the comparison between the SPF10 conventional and the real-time PCR results. In case of discrepancy, the samples were subjected to the CLART HPV2 assay. As a result, 27 out of 39 samples were identified as HPV-positive by the SPF10 real-time PCR and were genotyped further by the LiPA assay. Twenty samples (74.1%) showed an absolute agreement between the conventional and real-time SPF10 PCR (concordant), three (11.1%) displayed additional or fewer types (compatible), two (7.4%) did not show any similarity between both assays (discordant) and the remaining two (7.4%) were LiPA-negative. The two assays showed an excellent strength of agreement for individual (κ=0.932) and multiple genotype detection (κ=0.834). In conclusion, the two SPF10 PCR methods are comparable. Therefore, the SPF10 real-time PCR with subsequent LiPA could be used for the detection and genotyping of HPV in cervical samples.
新型 SPF10 实时 PCR 检测法可同时扩增和检测 HPV 靶标。这样，就可以避免对 HPV 阴性样本进行 LiPA 分析，从而减少工作量和成本。本研究旨在评估 SPF10 实时 PCR 联合 LiPA 检测法在宫颈样本 HPV 检测和分型中的性能。对 39 例宫颈样本进行 SPF10 常规 PCR 联合 LiPA 检测。随后，对 SPF10 实时 PCR 进行检测，以比较 SPF10 常规 PCR 和实时 PCR 的结果。如果结果不一致，则对样本进行 CLART HPV2 检测。结果，27 例 39 例样本经 SPF10 实时 PCR 鉴定为 HPV 阳性，并进一步用 LiPA 检测法进行基因分型。20 例（74.1%）在常规和实时 SPF10 PCR 之间显示出绝对一致性（一致），3 例（11.1%）显示出更多或更少的类型（相容），2 例（7.4%）在两种检测方法之间没有显示出任何相似性（不一致），其余 2 例（7.4%）LiPA 检测为阴性。两种检测方法在个体检测（κ=0.932）和多种基因型检测（κ=0.834）方面具有极好的一致性。总之，两种 SPF10 PCR 方法具有可比性。因此，可使用 SPF10 实时 PCR 联合随后的 LiPA 检测法用于宫颈样本的 HPV 检测和基因分型。
Zotero 插件