Micalessi M Isabel, Boulet Gaëlle A, Bogers Johannes
Applied Molecular Biology Research (AMBIOR) group, Laboratory of Cell Biology and Histology, Vaccine & Infectious Diseases Institute (VAXINFECTIO), University of Antwerp (Campus Groenenborger), Groenenborgerlaan 171, B-2020, Antwerp, Belgium,
Methods Mol Biol. 2015;1249:27-35. doi: 10.1007/978-1-4939-2013-6_2.
A highly sensitive SPF10 real-time PCR was developed to achieve simultaneous amplification and detection of the human papillomavirus (HPV) target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler(®) 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.
开发了一种高灵敏度的SPF10实时PCR，以实现人乳头瘤病毒（HPV）靶标的同时扩增和检测。通过这种方式，可以避免对HPV阴性样本进行线性探针分析（LiPA），从而减少工作量和成本。在此，我们详细描述了一种基于SYBR Green I的实时PCR检测方法，该方法使用LightCycler(®) 480系统，基于SPF10引物来生成和检测与LiPA检测兼容的HPV扩增子。
