溶组织内阿米巴对氧化剂的敏感性。
Susceptibility of Entamoeba histolytica to oxidants.
作者信息
Ghadirian E, Somerfield S D, Kongshavn P A
出版信息
Infect Immun. 1986 Jan;51(1):263-7. doi: 10.1128/iai.51.1.263-267.1986.
The effect of hydrogen peroxide and hypochlorite on culture forms of Entamoeba histolytica trophozoites was examined by using two strains of E. histolytica, virulent (IP:0682:1) and nonvirulent (DKB). The amoebae were incubated with various concentrations of hydrogen peroxide and hypochlorite, and their viability was determined at different times after incubation. When the viability of the virulent and nonvirulent strains was compared to different oxidant strengths, it became apparent that the virulent strain was less susceptible than the nonvirulent one to the cytotoxic effect of hydrogen peroxide and hypochlorite. Our studies further showed that the toxic effect was both time and dose dependent. To confirm that the killing of amoebae in this system was associated with the presence of hydrogen peroxide, amoebae were incubated with hydrogen peroxide and catalase. Catalase reduced the killing effect of hydrogen peroxide to the control level. These data confirmed previous observations of the susceptibility of amoebic trophozoites to hydrogen peroxide and also demonstrated susceptibility to hypochlorite.
通过使用两株溶组织内阿米巴，即强毒株（IP:0682:1）和无毒株（DKB），研究了过氧化氢和次氯酸盐对溶组织内阿米巴滋养体培养形式的影响。将阿米巴与不同浓度的过氧化氢和次氯酸盐一起孵育，并在孵育后的不同时间测定其活力。当比较强毒株和无毒株对不同氧化强度的活力时，很明显强毒株比无毒株对过氧化氢和次氯酸盐的细胞毒性作用更不敏感。我们的研究进一步表明，毒性作用具有时间和剂量依赖性。为了证实该系统中阿米巴的杀灭与过氧化氢的存在有关，将阿米巴与过氧化氢和过氧化氢酶一起孵育。过氧化氢酶将过氧化氢的杀灭作用降低到对照水平。这些数据证实了先前关于阿米巴滋养体对过氧化氢敏感性的观察结果，也证明了其对次氯酸盐的敏感性。
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