Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanjing Medical University, No.121 Jiang jia yuan, Nanjing, 210011, China.
Virchows Arch. 2013 Nov;463(5):713-9. doi: 10.1007/s00428-013-1475-4. Epub 2013 Aug 25.
Human trophoblastic cell surface antigen 2 (Trop2) has been suggested to play an important role in the development of solid tumors. However, the expression of Trop2 in extranodal NK/T cell lymphoma, nasal type (ENKTL) and the relationship with the clinical characteristics of this disease remain poorly understood. In this study, one-step quantitative PCR reverse transcription-polymerase chain reaction and immunohistochemical staining with tissue sections were employed to evaluate the expression of Trop2 in ENKTL. Furthermore, the relationship between Trop2 expression and prognosis of ENKTL was investigated. Expression of Trop2 mRNA and protein was significantly higher in ENKTL tissue than in corresponding non-lymphomatous tissue (p = 0.04 and p < 0.001, respectively). Expression of Trop2 protein in ENKTL was associated with lymph node involvement and poor overall survival (p = 0.045 and p = 0.018, respectively). Kaplan-Meier analysis and the logrank test indicated that lymph node involvement (p = 0.0481), single therapy strategy (p = 0.0037), and high expression of Trop2 (p = 0.0042) are significantly correlated with poor prognosis of ENKTL patients. The data suggest that Trop2 expression reflects a more malignant phenotype and may serve as an unfavorable prognostic factor for ENKTL.
人滋养层细胞表面抗原 2（Trop2）被认为在实体瘤的发生发展中发挥重要作用。然而，Trop2 在结外 NK/T 细胞淋巴瘤，鼻型（ENKTL）中的表达及其与该病临床特征的关系尚不清楚。在这项研究中，采用一步定量 PCR 逆转录-聚合酶链反应和组织切片免疫组织化学染色来评估 Trop2 在 ENKTL 中的表达。此外，还研究了 Trop2 表达与 ENKTL 预后的关系。Trop2 mRNA 和蛋白在 ENKTL 组织中的表达明显高于相应的非淋巴瘤组织（p=0.04 和 p<0.001）。Trop2 蛋白在 ENKTL 中的表达与淋巴结受累和总体生存不良相关（p=0.045 和 p=0.018）。Kaplan-Meier 分析和对数秩检验表明，淋巴结受累（p=0.0481）、单一治疗策略（p=0.0037）和 Trop2 高表达（p=0.0042）与 ENKTL 患者预后不良显著相关。这些数据表明 Trop2 表达反映了更具恶性表型的特征，可能是 ENKTL 的不良预后因素。
