Carr Ann L, Denning Steven S, Figurskey Anastasia C, Hung Kim Y, Reiskind Michael H, Wes Watson David
PathGroup, Nashville, TN, USA.
Department of Entomology & Plant Pathology, Box 7613, North Carolina State University, Raleigh, NC, USA.
J Insect Sci. 2025 Mar 14;25(2). doi: 10.1093/jisesa/ieaf020.
We developed a two-choice spatial olfactometer to evaluate the response of adult secondary screwworm (Cochliomyia macellaria), house fly (Musca domestica), and flesh fly (Sarcophaga bullata) to two commercially available fly-trap attractants, Captivator and FliesBeGone in three-dimensional space. Liquid fly baits were prepared according to the manufacturer's recommendations and aged to discern the relative attraction of fresh and older baits. Each 0.07 m3 (2.5 ft3) arena was fitted with two fresh air intake ports, collection chambers containing the attractant or a blank control, and air exhaust ports. We released adult flies into an arena with sufficient space to allow free flight and response to the test attractants. Each comparison was replicated eight times with fresh flies. Flies were more responsive to commercial bait than the water control. Air flowrates, as measured through the intake ports, was determined to be a limiting factor for C. macellaria and S. bullata with significant responses rates observed to flowrates ≤0.14 m3/min (5 ft3/min) and ≤0.25 m3/min (9 ft3/min), respectively. In contrast, M. domestica appeared to respond similarly to all flowrates tested (≤0.31 m3 (11 ft3/min). In direct comparisons with a water control, M. domestica was attracted to baits regardless of bait age. In similar experiments, C. macellaria was significantly responsive to FliesBeGone aged 2 and 3 d but not Captivator regardless of age. Lastly, S. bullata was most responsive to FliesBeGone aged 3 and 4 d, and Captivator aged 4 d. Female flies responded to fly baits more frequently than males.
我们开发了一种二选一空间嗅觉仪，用于评估成年次生螺旋蝇（嗜尸性麻蝇）、家蝇和肉蝇在三维空间中对两种市售捕蝇引诱剂Captivator和FliesBeGone的反应。液体蝇饵按照制造商的建议制备，并进行陈化处理，以辨别新鲜饵和陈旧饵的相对吸引力。每个0.07立方米（2.5立方英尺）的试验场装有两个新鲜空气入口、装有引诱剂或空白对照的收集室以及排气口。我们将成年苍蝇放入一个有足够空间的试验场，使其能够自由飞行并对测试引诱剂做出反应。每次比较用新鲜苍蝇重复进行八次。苍蝇对商业诱饵的反应比对水对照更敏感。通过进气口测量的气流速率被确定为嗜尸性麻蝇和肉蝇的限制因素，观察到当气流速率分别≤0.14立方米/分钟（5立方英尺/分钟）和≤0.25立方米/分钟（9立方英尺/分钟）时，它们有显著的反应率。相比之下，家蝇对所有测试的气流速率（≤0.31立方米（11立方英尺/分钟））的反应似乎相似。在与水对照的直接比较中，无论诱饵的陈化程度如何，家蝇都被诱饵吸引。在类似的实验中，嗜尸性麻蝇对陈化2天和3天的FliesBeGone有显著反应，但对Captivator无论陈化程度如何都无反应。最后，肉蝇对陈化3天和4天的FliesBeGone以及陈化4天的Captivator反应最为敏感。雌性苍蝇对蝇饵的反应比雄性更频繁。
