利用 CRISPR-Cas 在秀丽隐杆线虫中进行无转基因基因组编辑。
Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.
机构信息
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91125.
出版信息
Genetics. 2013 Nov;195(3):1167-71. doi: 10.1534/genetics.113.155879. Epub 2013 Aug 26.
Abstract
CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.
摘要
CRISPR-Cas 是一种从细菌到人类细胞的基因组编辑的有效方法。我们描述了一种无转基因的线虫 CRISPR-Cas 介导切割方法，该方法能够实现 RNA 同源靶向缺失，从而导致基因功能丧失；全基因组测序分析表明，该核酸酶活性具有高度特异性。
相似文献
1
Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.利用 CRISPR-Cas 在秀丽隐杆线虫中进行无转基因基因组编辑。
Genetics. 2013 Nov;195(3):1167-71. doi: 10.1534/genetics.113.155879. Epub 2013 Aug 26.
2
Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans.利用 Cas9-CRISPR 在秀丽隐杆线虫中靶向遗传突变和基因转换。
Genetics. 2013 Nov;195(3):1173-6. doi: 10.1534/genetics.113.155754. Epub 2013 Aug 26.
3
Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system.通过 CRISPR-Cas9 系统在秀丽隐杆线虫中进行可遗传的定制基因组修饰。
Genetics. 2013 Nov;195(3):1181-5. doi: 10.1534/genetics.113.156075. Epub 2013 Aug 26.
4
Exciting prospects for precise engineering of Caenorhabditis elegans genomes with CRISPR/Cas9.利用 CRISPR/Cas9 技术精确工程化秀丽隐杆线虫基因组的前景令人兴奋。
Genetics. 2013 Nov;195(3):635-42. doi: 10.1534/genetics.113.156521.
5
The application of CRISPR-Cas9 genome editing in Caenorhabditis elegans.CRISPR-Cas9基因组编辑技术在秀丽隐杆线虫中的应用。
J Genet Genomics. 2015 Aug 20;42(8):413-21. doi: 10.1016/j.jgg.2015.06.005. Epub 2015 Jun 26.
6
Approaches for CRISPR/Cas9 Genome Editing in C. elegans.秀丽隐杆线虫中 CRISPR/Cas9 基因组编辑的方法。
Methods Mol Biol. 2022;2468:215-237. doi: 10.1007/978-1-0716-2181-3_11.
7
Precise and heritable genome editing in evolutionarily diverse nematodes using TALENs and CRISPR/Cas9 to engineer insertions and deletions.使用 TALENs 和 CRISPR/Cas9 在进化上多样化的线虫中进行精确且可遗传的基因组编辑，以工程插入和缺失。
Genetics. 2013 Oct;195(2):331-48. doi: 10.1534/genetics.113.155382. Epub 2013 Aug 9.
8
Rapid and precise engineering of the Caenorhabditis elegans genome with lethal mutation co-conversion and inactivation of NHEJ repair.利用致死突变共转化和非同源末端连接（NHEJ）修复失活对秀丽隐杆线虫基因组进行快速精确工程改造。
Genetics. 2015 Feb;199(2):363-77. doi: 10.1534/genetics.114.172361. Epub 2014 Dec 9.
9
The application of somatic CRISPR-Cas9 to conditional genome editing in Caenorhabditis elegans.体细胞CRISPR-Cas9在秀丽隐杆线虫条件性基因组编辑中的应用。
Genesis. 2016 Apr;54(4):170-81. doi: 10.1002/dvg.22932. Epub 2016 Apr 14.
10
Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System.利用CRISPR/Cas9系统在秀丽隐杆线虫中创建基因组修饰
Methods Mol Biol. 2015;1327:59-74. doi: 10.1007/978-1-4939-2842-2_6.
引用本文的文献
1
The transsulfuration pathway suppresses the embryonic lethal phenotype of glutathione reductase mutants in Caenorhabditis elegans.转硫途径抑制秀丽隐杆线虫中谷胱甘肽还原酶突变体的胚胎致死表型。
G3 (Bethesda). 2025 Aug 6;15(8). doi: 10.1093/g3journal/jkaf102.
2
Genome editing using CRISPR, CAST, and Fanzor systems.使用 CRISPR、CAST 和 Fanzor 系统进行基因组编辑。
Mol Cells. 2024 Jul;47(7):100086. doi: 10.1016/j.mocell.2024.100086. Epub 2024 Jun 21.
3
A nematode model to evaluate microdeletion phenotype expression.评估微缺失表型表达的线虫模型。
G3 (Bethesda). 2024 Feb 7;14(2). doi: 10.1093/g3journal/jkad258.
4
Integrating non-mammalian model organisms in the diagnosis of rare genetic diseases in humans.将非哺乳动物模式生物应用于人类罕见遗传病的诊断
Nat Rev Genet. 2024 Jan;25(1):46-60. doi: 10.1038/s41576-023-00633-6. Epub 2023 Jul 25.
5
A Decade of CRISPR-Cas Gnome Editing in .十年的 CRISPR-Cas Gnome 编辑。
Int J Mol Sci. 2022 Dec 14;23(24):15863. doi: 10.3390/ijms232415863.
6
Highly efficient transgenesis with miniMos in Caenorhabditis briggsae.利用 miniMos 在秀丽隐杆线虫中进行高效的转基因。
G3 (Bethesda). 2022 Dec 1;12(12). doi: 10.1093/g3journal/jkac254.
7
Clustered Regularly Interspaced Short Palindromic Repeats/ CRISPR associated protein 9-mediated editing of Schistosoma mansoni genes: Identifying genes for immunologically potent drug and vaccine development.丛集规律间隔短回文重复序列/ CRISPR 相关蛋白 9 介导的曼氏血吸虫基因编辑：鉴定具有免疫效力的药物和疫苗开发基因。
Rev Soc Bras Med Trop. 2022 Aug 12;55:e0131. doi: 10.1590/0037-8682-0131-2022. eCollection 2022.
8
Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.模块化安全港转基因插入用于靶向单拷贝和额外染色体阵列在秀丽隐杆线虫中的整合。
G3 (Bethesda). 2022 Aug 25;12(9). doi: 10.1093/g3journal/jkac184.
9
Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.使用光学显微镜观察和量化秀丽隐杆线虫中的分子和细胞过程。
Genetics. 2022 Jul 30;221(4). doi: 10.1093/genetics/iyac068.
10
Modified Gene Editing Systems: Diverse Bioengineering Tools and Crop Improvement.改良基因编辑系统：多样的生物工程工具与作物改良
Front Plant Sci. 2022 Mar 17;13:847169. doi: 10.3389/fpls.2022.847169. eCollection 2022.
本文引用的文献
1
Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination.利用 Cas9 触发的同源重组工程化秀丽隐杆线虫基因组。
Nat Methods. 2013 Oct;10(10):1028-34. doi: 10.1038/nmeth.2641. Epub 2013 Sep 1.
2
CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans.CRISPR/Cas9 在秀丽隐杆线虫中的靶向突变。
Genetics. 2013 Nov;195(3):1187-91. doi: 10.1534/genetics.113.156299. Epub 2013 Aug 26.
3
Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system.通过 CRISPR-Cas9 系统在秀丽隐杆线虫中进行可遗传的定制基因组修饰。
Genetics. 2013 Nov;195(3):1181-5. doi: 10.1534/genetics.113.156075. Epub 2013 Aug 26.
4
Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans.利用 Cas9-CRISPR 在秀丽隐杆线虫中靶向遗传突变和基因转换。
Genetics. 2013 Nov;195(3):1173-6. doi: 10.1534/genetics.113.155754. Epub 2013 Aug 26.
5
Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins.通过直接注射 Cas9-sgRNA 核糖核蛋白在秀丽隐杆线虫中进行可遗传的基因敲除。
Genetics. 2013 Nov;195(3):1177-80. doi: 10.1534/genetics.113.155853. Epub 2013 Aug 26.
6
Precise and heritable genome editing in evolutionarily diverse nematodes using TALENs and CRISPR/Cas9 to engineer insertions and deletions.使用 TALENs 和 CRISPR/Cas9 在进化上多样化的线虫中进行精确且可遗传的基因组编辑，以工程插入和缺失。
Genetics. 2013 Oct;195(2):331-48. doi: 10.1534/genetics.113.155382. Epub 2013 Aug 9.
7
Heritable genome editing in C. elegans via a CRISPR-Cas9 system.利用 CRISPR-Cas9 系统对秀丽隐杆线虫进行可遗传的基因组编辑。
Nat Methods. 2013 Aug;10(8):741-3. doi: 10.1038/nmeth.2532. Epub 2013 Jun 30.
8
Molecular characterisation of the recovery process in the entomopathogenic nematode Heterorhabditis bacteriophora.昆虫病原线虫嗜菌异小杆线虫复苏过程的分子特征。
Int J Parasitol. 2013 Sep;43(10):843-52. doi: 10.1016/j.ijpara.2013.05.009. Epub 2013 Jun 24.
9
The million mutation project: a new approach to genetics in Caenorhabditis elegans.百万突变体项目：秀丽隐杆线虫遗传学的新方法。
Genome Res. 2013 Oct;23(10):1749-62. doi: 10.1101/gr.157651.113. Epub 2013 Jun 25.
10
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.CRISPR-Cas 核酸酶在人类细胞中诱导的高频脱靶突变。
Nat Biotechnol. 2013 Sep;31(9):822-6. doi: 10.1038/nbt.2623. Epub 2013 Jun 23.
Zotero 插件