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濒危海洋鱼类波纹唇鱼(苏眉鱼)微卫星位点的开发与特征分析

Development and characterization of microsatellite loci in a threatened marine fish, Cheilinus undulatus (humphead wrasse).

作者信息

Hu J, Zhu X P, Luo J, Yin S W, Peng Y H, Hu Y L, Zhu F

机构信息

College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, China.

出版信息

Genet Mol Res. 2013 Jul 30;12(3):2633-6. doi: 10.4238/2013.July.30.2.

DOI:10.4238/2013.July.30.2
PMID:23979889
Abstract

Cheilinus undulatus (humphead wrasse) is a marine fish distributed widely throughout the tropical Indo-Pacific. It has been listed as vulnerable in the IUCN Red Data Book and in CITES Appendix II four times. Fifteen microsatellite loci were isolated and characterized for this species. The number of alleles ranged from 3 to 15 per locus, and the observed and expected heterozygosity ranged between 0.0323-0.7742 and 0.2597-0.8773, respectively. The polymorphism information content ranged from 0.2353-0.8520. Four microsatellite loci deviated significantly from Hardy-Weinberg expectations. No significant linkage disequilibrium was found among any of the loci. These microsatellite loci will be useful for future investigations of genetic variation in the wrasse population.

摘要

波纹唇鱼是一种广泛分布于热带印度-太平洋地区的海洋鱼类。它已被列入国际自然保护联盟红色名录中的易危物种,并四次被列入《濒危野生动植物种国际贸易公约》附录二。为该物种分离并鉴定了15个微卫星位点。每个位点的等位基因数在3至15个之间,观察到的杂合度和预期杂合度分别在0.0323 - 0.7742和0.2597 - 0.8773之间。多态信息含量在0.2353 - 0.8520之间。四个微卫星位点显著偏离哈迪-温伯格平衡预期。在任何位点之间均未发现显著的连锁不平衡。这些微卫星位点将有助于未来对波纹唇鱼种群遗传变异的研究。

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