Bai C-C, Liu S-F, Zhuang Z-M, Yuan Y-J, Liu H-B, Dai F-Q
Key Laboratory for Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Genet Mol Res. 2012 May 18;11(2):1465-8. doi: 10.4238/2012.May.18.7.
Synechogobius hasta is an important commercial marine fish with distinctive features of rapid growth and short lifespan. We isolated and characterized 17 microsatellite markers for S. hasta using a (GT)(13)-enriched genomic library. Polymorphism was assessed in 48 individuals from a single population collected from the northern coastal waters of the Yellow Sea. The number of alleles per locus ranged from 2 to 23, with a mean of 11.3. The observed and expected heterozygosities ranged from 0.130 to 1.000 and from 0.123 to 0.939, with means of 0.758 and 0.774, respectively. Fourteen of 17 loci conformed to Hardy-Weinberg equilibrium and no significant linkage disequilibrium between locus pairs was detected. These microsatellite markers will be useful for population genetic structure analyses.
矛尾复虾虎鱼是一种重要的商业性海洋鱼类，具有生长迅速和寿命短的显著特点。我们利用一个（GT）(13)富集基因组文库，分离并鉴定了17个矛尾复虾虎鱼的微卫星标记。对从黄海北部沿海水域采集的一个单一种群的48个个体进行了多态性评估。每个位点的等位基因数范围为2至23，平均为11.3。观察到的杂合度和预期杂合度范围分别为0.130至1.000和0.123至0.939，平均值分别为0.758和0.774。17个位点中的14个符合哈迪-温伯格平衡，未检测到位点对之间存在显著的连锁不平衡。这些微卫星标记将有助于进行种群遗传结构分析。
