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Characterization of new microsatellite markers of Siganus fuscescens (Siganidae).

作者信息

Li Q H, Li Z B, Dai G, Chen X J, Chen L N, Cao Y Y, Shangguan J B, Ning Y F

机构信息

Fisheries College, Jimei University, Xiamen, China.

出版信息

Genet Mol Res. 2013 Jul 30;12(3):2751-4. doi: 10.4238/2013.July.30.12.

DOI:10.4238/2013.July.30.12
PMID:23979899
Abstract

Siganus fuscescens, which is a small commercially important marine fish, is wildly distributed in shallow waters throughout the tropical and subtropical Indo-Pacific and Eastern Mediterranean regions. It is part of a group known as rabbitfish. Fifteen new polymorphic microsatellite markers for S. fuscescens were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 2 to 12, and the polymorphism information content ranged from 0.210 to 0.849. The observed and expected heterozygosities were 0.142-0.808 and 0.225-0.853, respectively. Although significant deviations from Hardy-Weinberg equilibrium were detected at 2 loci (Sf1-37-2 and Sf1-47), no significant deviations were detected at the other 13 loci. These microsatellite markers will provide a useful tool for studies on genetic diversity and differentiation of S. fuscescens.

摘要

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