Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Thiruvananthapuram 695012, India.
J Biomed Nanotechnol. 2013 Sep;9(9):1483-94. doi: 10.1166/jbn.2013.1640.
The present work is a comparative evaluation of physical and biological properties of electrospun biodegradable fibrous scaffolds based on polycaprolactone (PCL) and its blend with polycaprolactone-polyethyleneglycol-polycaprolactone (CEC) with and without nanohydroxyapatite (nHAP) particles. The fiber morphology, porosity, surface wettability, and mechanical properties of electrospun PCL were distinctly influenced by the presence of both copolymer CEC and nHAP. The degradation in hydrolytic media affected both morphological and mechanical properties of the scaffolds and the tensile strength decreased by 58% for PCL, 83% for PCL/CEC, 36% for PCL/nHAP and 75% for PCL/CEC/nHAP in 90 days of PBS ageing. MTT assay using mouse fibroblast L929 cells proved all the scaffolds to be non-cytotoxic. An overall enhanced performance was shown by PCL/CEC/nHAP scaffold in cell viability (LPH) and proliferation (Picogreen). Simultaneously, ELF assay of ALP activity (bone marker) confirmed the presence of osteogenic-induced Rabbit adipose-derived mesenchymal stem cells (ADMSCs) on all the scaffolds. In comparison, the results reveal the potential of the cytocompatible PCL/CEC/nHAP scaffold for the fabrication of living bony constructs for tissue engineering applications.
本工作对基于聚己内酯（PCL）的电纺生物可降解纤维支架的物理和生物性能进行了比较评估，并将其与聚己内酯-聚乙二醇-聚己内酯（CEC）共混物进行了比较，其中分别含有和不含有纳米羟基磷灰石（nHAP）颗粒。纤维形态、孔隙率、表面润湿性和机械性能均明显受到共聚物 CEC 和 nHAP 的存在的影响。在水解介质中的降解同时影响支架的形态和机械性能，并且在 PBS 老化 90 天时，PCL 的拉伸强度降低了 58%，PCL/CEC 降低了 83%，PCL/nHAP 降低了 36%，PCL/CEC/nHAP 降低了 75%。使用小鼠成纤维细胞 L929 细胞进行的 MTT 测定证明所有支架均无细胞毒性。PCL/CEC/nHAP 支架在细胞活力（LPH）和增殖（Picogreen）方面表现出整体增强的性能。同时，碱性磷酸酶（骨标志物）的 ELF 测定证实所有支架上均存在诱导成骨的兔脂肪间充质干细胞（ADMSCs）。相比之下，结果表明，该细胞相容性 PCL/CEC/nHAP 支架具有用于组织工程应用的生物活性骨构建体的制造潜力。
