Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Sao Paulo State University, 12245-000, Sao Jose dos Campos, Sao Paulo, Brazil.
Instituto Científico e Tecnológico, Universidade Brasil, 08230-030, Sao Paulo, Brazil.
J Mater Sci Mater Med. 2020 Jul 27;31(8):72. doi: 10.1007/s10856-020-06403-8.
Polycaprolactone (PCL) is a biocompatible, biodegradable synthetic polymer which in combination with nanohydroxyapatite (nHAp) can give rise to a low cost, nontoxic bioactive product with excellent mechanical properties and slow degradation. Here we produced, characterized and evaluated in vivo the bone formation of PCL/nHAp scaffolds produced by the rotary jet spinning technique. The scaffolds produced were firstly soaked into simulated body fluid for 21 days to also obtain nHAp onto PCL/nHAp scaffolds. Afterwards, the scaffolds were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy and Raman spectroscopy. For in vivo experiments, 20 male Wistar rats were used and randomly divided in 4 experimental groups (n = 5). A critical defect of 3 mm in diameter was made in the tibia of the animals, which were filled with G1 control (clot); G2-PCL scaffold; G3-PCL/nHAp (5%) scaffold; G4-PCL/nHAp (20%) scaffold. All animals were euthanized 60 days after surgery, and the bone repair in the right tibiae were evaluated by radiographic analysis, histological analysis and histomorphometric analysis. While in the left tibias, the areas of bone repair were submitted to the flexural strength test. Radiographic and histomorphometric analyses no showed statistical difference in new bone formation between the groups, but in the three-point flexural tests, the PCL/nHAp (20%) scaffold positively influenced the flexural mode of the neoformed bone. These findings indicate that PCL/nHAp (20%) scaffold improve biomechanical properties of neoformed bone and could be used for bone medicine regenerative.
聚己内酯（PCL）是一种生物相容、可生物降解的合成聚合物，与纳米羟基磷灰石（nHAp）结合可以产生具有优异机械性能和缓慢降解的低成本、无毒的生物活性产品。在这里，我们通过旋转喷射纺丝技术生产、表征和评估了PCL/nHAp 支架的体内骨形成。首先将制备的支架浸泡在模拟体液中 21 天，以使 nHAp 也沉积在 PCL/nHAp 支架上。然后，通过扫描电子显微镜（SEM）、能量色散光谱和拉曼光谱对支架进行了表征。在体内实验中，使用 20 只雄性 Wistar 大鼠，随机分为 4 个实验组（n=5）。在动物的胫骨上制造 3mm 直径的临界缺陷，并用 G1 对照组（血凝块）；G2-PCL 支架；G3-PCL/nHAp（5%）支架；G4-PCL/nHAp（20%）支架填充。所有动物均在手术后 60 天处死，通过放射分析、组织学分析和组织形态计量学分析评估右胫骨的骨修复情况。而在左胫骨中，将骨修复区域进行三点弯曲强度测试。放射学和组织形态计量学分析未显示各组之间新骨形成的统计学差异，但在三点弯曲试验中，PCL/nHAp（20%）支架对新形成骨的弯曲模式产生了积极影响。这些发现表明，PCL/nHAp（20%）支架可改善新形成骨的生物力学性能，并可用于骨再生医学。
