Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre,
J Biomed Nanotechnol. 2013 Sep;9(9):1521-31. doi: 10.1166/jbn.2013.1647.
Poor bioavailability of antifungal drugs due to the various protective mechanisms of the eye is a serious concern for the treatment of corneal fungal infections in today's world. The use of nanosystems that can improve the bioavailability of these antifungal drugs is relatively a new idea being conceived and here we have synthesized fluconazole loaded chitin nanogels (Flu-CNGs) which can be used for the treatment of corneal fungal infections. These nanogels were characterized using DLS, Zeta potential, SEM, FTIR and TG/DTA. The prepared Flu-CNGs have controlled release pattern which is ideal for the continuous availability of fluconazole over a longer period of time for an effective fungal treatment. Flu-CNGs are haemocompatible, cytocompatible and also showed very good cell uptake in human dermal fibroblast cells and penetration to the deeper sections of the porcine cornea with no signs of destruction or inflammation to corneal cells as shown in ex vivo permeation studies.
由于眼睛的各种保护机制，抗真菌药物的生物利用度较差，这是当今治疗角膜真菌感染的一个严重问题。使用纳米系统可以提高这些抗真菌药物的生物利用度，这是一个相对较新的想法，在这里我们合成了负载氟康唑的壳聚糖纳米凝胶（Flu-CNGs），可用于治疗角膜真菌感染。这些纳米凝胶使用 DLS、Zeta 电位、SEM、FTIR 和 TG/DTA 进行了表征。制备的 Flu-CNGs 具有控制释放模式，非常适合在较长时间内持续提供氟康唑，以实现有效的真菌治疗。Flu-CNGs 具有血液相容性、细胞相容性，并且在人皮肤成纤维细胞中表现出非常好的细胞摄取，并且在没有角膜细胞破坏或炎症迹象的情况下穿透猪角膜的深层部位，如在体外渗透研究中所示。
