一只脾切除小牛的脑心肌炎病毒感染

Encephalomyocarditis virus infection in a splenectomised calf.

作者信息

Diallo I S, Carter P D, Storie G

机构信息

Biosecurity Sciences Laboratory, Health and Food Science Precinct, Department of Agriculture, Fisheries and Forestry, PO Box 156, Archerfield BC, Queensland, 4108, Australia.

出版信息

Aust Vet J. 2013 Sep;91(9):391-4. doi: 10.1111/avj.12096.

DOI:10.1111/avj.12096

PMID:23980833

Abstract

BACKGROUND

Encephalomyocarditis (EMC) caused by EMC virus (EMCV) was diagnosed in a 5-month-old splenectomised calf, which died suddenly on an experimental farm that had a high infestation of rodents.

RESULTS

At postmortem examination, the lungs were dark purple and diffusely congested. On histological examination, the calf had severe necrotising myocarditis. EMCV was isolated from the heart. The polyprotein gene of the EMCV isolate was amplified by PCR and had 85-91% identity with published EMCV sequences, including 89% identity with isolates from Queensland. On phylogenetic analysis, the polyprotein gene had highest sequence identity with South Korean EMCV strain, CBNU.

CONCLUSION

This is the first report of naturally occurring EMC in cattle in Australia and the first report of naturally occurring bovine EMC from which EMCV has been isolated.

摘要

背景

在一头5个月大的脾切除小牛中诊断出由脑心肌炎病毒（EMCV）引起的脑心肌炎（EMC），该小牛在一个啮齿动物大量滋生的实验农场突然死亡。

结果

尸检时，肺呈深紫色且弥漫性充血。组织学检查显示，该小牛患有严重的坏死性心肌炎。从心脏中分离出了EMCV。通过聚合酶链反应（PCR）扩增了EMCV分离株的多聚蛋白基因，其与已发表的EMCV序列具有85% - 91%的同一性，其中与来自昆士兰的分离株具有89%的同一性。系统发育分析表明，多聚蛋白基因与韩国EMCV毒株CBNU具有最高的序列同一性。