Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, 100193, China.
Sci Rep. 2017 Mar 27;7(1):438. doi: 10.1038/s41598-017-00435-x.
Encephalomyocarditis virus (EMCV) is as a potential zoonotic agent with a wide host range. Here, we describe an EMC virus isolate, identified as EMCV C15, which was successfully obtained from the serum of dogs from animal hospitals. Virus production in cell culture was confirmed by EMCV-specific real-time RT-PCR, indirect immunofluorescence assays and electron microscopy. In addition, the open reading frame sequence (ORF) of the EMCV C15 virus was determined. From sequence comparison and phylogenetic analysis among 24 reference EMCV strains, it appears that the EMCV C15 strain is closely genetically related to strain BEL2887A/91 (>99.0% nucleotide identity). In artificially challenged dogs, the heart and brain were important targets of EMCV C15. This study provides genetic and pathogenic characterization of the EMCV C15 strain isolated in Beijing and calls for sustained surveillance of EMCV infection in China to support better prevention and control of the disease.
脑心肌炎病毒（EMCV）是一种具有广泛宿主范围的潜在人畜共患病病原体。在这里，我们描述了一种 EMC 病毒分离株，称为 EMCV C15，它是从动物医院的狗血清中成功获得的。通过 EMCV 特异性实时 RT-PCR、间接免疫荧光检测和电子显微镜观察确认了细胞培养中的病毒产生。此外，还确定了 EMCV C15 病毒的开放阅读框序列（ORF）。通过对 24 株参考 EMCV 株的序列比较和系统发育分析，EMCV C15 株与 BEL2887A/91 株密切相关（>99.0%核苷酸同源性）。在人工攻毒的狗中，心脏和大脑是 EMCV C15 的重要靶标。本研究提供了在北京分离的 EMCV C15 株的遗传和致病性特征，并呼吁对中国的 EMCV 感染进行持续监测，以支持更好地预防和控制该疾病。
