Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University , Changchun, Jilin , People's Republic of China .
Immunopharmacol Immunotoxicol. 2013 Oct;35(5):615-21. doi: 10.3109/08923973.2013.830126. Epub 2013 Aug 28.
Gossypol is a kind of yellow polyphenolic compounds extracted from root stem and seed of the cotton plant. In the present study, we investigated its immunosuppressive mechanism by using BALB/c mouse T lymphocytes in vitro. When mouse splenocytes was incubated with gossypol, the extract effectively suppress the overproduction of the cell stimulated by concanavalin A (ConA) in a dose manner. This inhibitive activity was mainly due to interfering Th1 and Th2 cytokines production and decreasing CD4(+) T cell populations and ratio of CD4(+)/CD8(+). Furthermore, we also showed that signal transduction via NF-κB, NFAT and AP-1 are critical to the ConA-induced T cell activation in mice. The data revealed that gossypol could down-regulate activation of ConA-induced NF-κB, NFAT and AP-1 signal transduction pathways in mouse T lymphocyte. These observations indicated that gossypol exhibited immunosuppressive effects by inhibition T lymphocyte activation in vitro.
棉酚是从棉属植物的根、茎和种子中提取的一种黄色多酚化合物。本研究采用体外培养的 BALB/c 小鼠 T 淋巴细胞，探讨了其免疫抑制作用的机制。当用棉酚孵育小鼠脾细胞时，该提取物可有效抑制刀豆蛋白 A（ConA）刺激的细胞过度产生，呈剂量依赖性。这种抑制活性主要是由于干扰 Th1 和 Th2 细胞因子的产生，减少 CD4+T 细胞的数量和 CD4+/CD8+的比例。此外，我们还表明，NF-κB、NFAT 和 AP-1 信号转导途径对 ConA 诱导的小鼠 T 细胞激活至关重要。研究结果表明，棉酚可下调 ConA 诱导的 NF-κB、NFAT 和 AP-1 信号转导通路在小鼠 T 淋巴细胞中的激活。这些结果表明，棉酚通过抑制 T 淋巴细胞的体外激活表现出免疫抑制作用。
