Bégin M E, Ells G, St-Jean P, Vachereau A, Beaudoin A R
Centre de recherche sur les méchanismes de sécrétion, Faculté des Sciences, Université de Sherbrooke, Québec, Canada.
Int J Pancreatol. 1990 Apr;6(3):151-60. doi: 10.1007/BF02924285.
The influence of n-3 and n-6 PUFA on the fatty acid composition and the enzyme content of zymogen granules of the normal exocrine pancreas was tested on rats. The animals were fed on different diets comprising 5% fish oil (FO), safflower oil (SFO), and evening primrose oil (EPO) used singly or in combination as dietary fats. The results were compared with those from animals fed 5% hydrogenated beef tallow (HBT). The fatty acid composition and digestive enzyme content were analyzed after a 6-wk feeding period. Differences in the pancreatic fatty acid profiles were related to the fatty acid composition of the ingested fats. Equivalent levels of n-3 fatty acids and 20:3n-6 were obtained with either EPO or FO fed singly or in combination. Similar results were observed with SFO/FO. Higher C20:3n-6/C20:4n-6 ratios were obtained with the oil mixtures. An increase in amylase levels, but a decrease in serine protease (Band 21 kdalton) levels, was associated with EPO. An elevation in procarboxypeptidase levels paralleled an increase in 18:0 levels, whereas the proportion of lipase (Band 49 kdalton) varied inversely with the proportion of C20:3n-6. The SFO/FO mixture elevated the proportions of protease II and proelastase. These results suggest that specific fatty acids influence the proportion of specific digestive enzymes in the zymogen granules.
在大鼠身上测试了n-3和n-6多不饱和脂肪酸（PUFA）对正常外分泌胰腺酶原颗粒脂肪酸组成和酶含量的影响。给动物喂食不同的饮食，其中分别或组合使用5%的鱼油（FO）、红花油（SFO）和月见草油（EPO）作为膳食脂肪。将结果与喂食5%氢化牛油（HBT）的动物的结果进行比较。在6周的喂食期后分析脂肪酸组成和消化酶含量。胰腺脂肪酸谱的差异与摄入脂肪的脂肪酸组成有关。单独或组合喂食EPO或FO可获得相当水平的n-3脂肪酸和20:3n-6。SFO/FO组合也观察到类似结果。油混合物的C20:3n-6/C20:4n-6比值更高。EPO与淀粉酶水平升高但丝氨酸蛋白酶（21 kDa条带）水平降低有关。羧肽酶原水平的升高与18:0水平的升高平行，而脂肪酶（49 kDa条带）的比例与C20:3n-6的比例呈负相关。SFO/FO混合物提高了蛋白酶II和前弹性蛋白酶的比例。这些结果表明，特定脂肪酸会影响酶原颗粒中特定消化酶的比例。
