Malihezaman Monsefi, Mojaba Masoudi, Elham Hosseini, Farnaz Gramifar, Ramin Miri
Biology Department, College of Sciences, Shiraz University, Shiraz-Iran.
Afr J Tradit Complement Altern Med. 2012 Apr 2;9(3):336-41. doi: 10.4314/ajtcam.v9i3.6. eCollection 2012.
Our previous studies showed the effects of aqueous and ethanol extracts of Anethum graveolens L. (dill) on female infertility. In the present study we investigated whether different fractions of this herb extract can cause infertility in rats. Female rats were divided into the control groups, the groups receiving either a low (0.5 g/kg)) or a high dose (5g/kg) of water, N-butanol, chloroform and ether fractions of the aqueous plant extract, and the groups receiving either a low (0.045 g/kg) or a high dose (0.45 g/kg) of the same fractions of ethanol extract. The mentioned doses were gavaged in 1mL for 10 days. Vaginal smears were prepared daily. Estradiol and progesterone levels were measured. The left oviduct and ovary were removed, their tissue subsequently being prepared in form of histology slides and stained using haematoxylin-eosin and Masson's trichrome. Female rats assigned to each group were mated with males; after that, crown-rump lengths and weights of newborn rats were measured. Results showed that each fraction produced some changes such as hormonal level reduction (chloroform fraction), diestrus phase prolongation and infertility (water fraction), and increase in pregnancy duration (chloroform and ether fractions). We concluded that each fraction comprises only some of the mentioned components and therefore recommended the usage of crude extract, especially the aqueous one, in case infertility aims to be induced.
我们之前的研究表明了莳萝水提取物和乙醇提取物对雌性不育的影响。在本研究中，我们调查了这种草药提取物的不同馏分是否会导致大鼠不育。将雌性大鼠分为对照组、接受低剂量（0.5 g/kg）或高剂量（5 g/kg）的水、正丁醇、氯仿和醚馏分的水植物提取物的组，以及接受低剂量（0.045 g/kg）或高剂量（0.45 g/kg）的相同馏分乙醇提取物的组。上述剂量以1 mL灌胃，持续10天。每天制备阴道涂片。测量雌二醇和孕酮水平。切除左侧输卵管和卵巢，随后将其组织制成组织学切片，并使用苏木精-伊红和马松三色染色法进行染色。将每组的雌性大鼠与雄性大鼠交配；之后，测量新生大鼠的顶臀长度和体重。结果表明，每个馏分都产生了一些变化，如激素水平降低（氯仿馏分）、动情间期延长和不育（水馏分），以及妊娠期延长（氯仿和醚馏分）。我们得出结论，每个馏分仅包含上述部分成分，因此建议在旨在诱导不育的情况下使用粗提取物，尤其是水提取物。
