Seo Hyeon-Beom, Kim Seungseop, Lee Hyeon-Yong, Jung Kyung-Hwan
Division of Food and Biotechnology, Chungju National University, Jeungpyung, Chungbuk 368-701, Korea.
Mycobiology. 2009 Jun;37(2):133-40. doi: 10.4489/MYCO.2009.37.2.133. Epub 2009 Jun 30.
To optimally convert corn hull, a byproduct from corn processing, into bioethanol using Pachysolen tannophlius, we investigated the optimal conditions for hydrolysis and removal of toxic substances in the hydrolysate via activated carbon treatment as well as the effects of this detoxification process on the kinetic parameters of bioethanol production. Maximum monosaccharide concentrations were obtained in hydrolysates in which 20 g of corn hull was hydrolyzed in 4% (v/v) H2SO4. Activated carbon treatment removed 92.3% of phenolic compounds from the hydrolysate. When untreated hydrolysate was used, the monosaccharides were not completely consumed, even at 480 h of culture. When activated carbon-treated hydrolysate was used, the monosaccharides were mostly consumed at 192 h of culture. In particular, when activated carbon-treated hydrolysate was used, bioethanol productivity (P) and specific bioethanol production rate (Qp) were 2.4 times and 3.4 times greater, respectively, compared to untreated hydrolysate. This was due to sustained bioethanol production during the period of xylose/arabinose utilization, which occurred only when activated carbon-treated hydrolysate was used.
为了利用嗜鞣管囊酵母将玉米加工副产品玉米皮最佳地转化为生物乙醇，我们研究了水解的最佳条件以及通过活性炭处理去除水解产物中有毒物质的方法，同时研究了这种解毒过程对生物乙醇生产动力学参数的影响。在含有20 g玉米皮且用4%（v/v）硫酸水解的水解产物中获得了最大单糖浓度。活性炭处理从水解产物中去除了92.3%的酚类化合物。当使用未处理的水解产物时，即使在培养480小时后，单糖也没有完全消耗。当使用活性炭处理的水解产物时，单糖在培养192小时时大部分被消耗。特别是，当使用活性炭处理的水解产物时，生物乙醇生产率（P）和比生物乙醇生产速率（Qp）分别比未处理的水解产物高2.4倍和3.4倍。这是由于仅在使用活性炭处理的水解产物时，在木糖/阿拉伯糖利用期间生物乙醇持续生产。
