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利用重复和连续诱变改进纤维素酶生产的真菌菌株。

Fungal strain improvement for cellulase production using repeated and sequential mutagenesis.

作者信息

Vu Van Hanh, Pham Tuan Anh, Kim Keun

机构信息

Department of Bioscience and Biotechnology, The University of Suwon, Hwaseong 445-743, Korea.

出版信息

Mycobiology. 2009 Dec;37(4):267-71. doi: 10.4489/MYCO.2009.37.4.267. Epub 2009 Dec 31.

DOI:10.4489/MYCO.2009.37.4.267
PMID:23983546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3749416/
Abstract

A fungal strain producing a high level of cellulase was selected from 320 fungal isolates and identified as Aspergillus sp. This strain was further improved for cellulase production by sequential treatments by two repeated rounds of γ-irradiation of Co(60), ultraviolet treatment and four repeated rounds of treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The best mutant strain, Aspergillus sp. XTG-4, was selected after screening and the activities of carboxymethyl cellulase, filter paper cellulase and β-glucosidase of the cellulase were improved by 2.03-, 3.20-, and 1.80-fold, respectively, when compared to the wild type strain. After being subcultured 19 times, the enzyme production of the mutant Aspergillus sp. XTG-4s was stable.

摘要

从320株真菌分离物中筛选出一株高产纤维素酶的真菌菌株，并鉴定为曲霉属。通过两轮重复的钴（60）γ射线辐照、紫外线处理以及四轮重复的N-甲基-N'-硝基-N-亚硝基胍处理对该菌株进行连续处理，进一步提高其纤维素酶产量。筛选后得到最佳突变菌株曲霉属XTG-4，与野生型菌株相比，其纤维素酶的羧甲基纤维素酶、滤纸纤维素酶和β-葡萄糖苷酶活性分别提高了2.03倍、3.20倍和1.80倍。突变菌株曲霉属XTG-4传代培养19次后，产酶稳定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee0/3749416/d8e8900f43b4/mb-37-267-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee0/3749416/2226fac03168/mb-37-267-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee0/3749416/521cbbd337e3/mb-37-267-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee0/3749416/d8e8900f43b4/mb-37-267-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee0/3749416/2226fac03168/mb-37-267-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee0/3749416/521cbbd337e3/mb-37-267-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee0/3749416/d8e8900f43b4/mb-37-267-g003.jpg

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