绿色木霉的纤维素酶。参与棉纤维溶解的成分的分离。
The cellulase of Trichoderma viride. Separation of the components involved in the solubilization of cotton.
作者信息
Selby K, Maitland C C
出版信息
Biochem J. 1967 Sep;104(3):716-24. doi: 10.1042/bj1040716.
Abstract
- Culture filtrates from Trichoderma viride have been fractionated by gel filtration on Sephadex G-75 followed by ion-exchange chromatography on DEAE- and SE-Sephadex. 2. The components essential for attack on cotton are a carboxymethylcellulase, a cellobiase and a third (C(1)) component which has no action on CM-cellulose, cellobiose or cotton. 3. These components, which together can completely convert cotton into water-soluble products, lose this ability when separated and regain it quantitatively when recombined in their original proportions.
摘要
- 绿色木霉的培养滤液先用葡聚糖凝胶G-75进行凝胶过滤，然后在DEAE-葡聚糖凝胶和SE-葡聚糖凝胶上进行离子交换色谱分离。2. 攻击棉花所必需的成分是一种羧甲基纤维素酶、一种纤维二糖酶和第三种（C(1)）成分，该成分对羧甲基纤维素、纤维二糖或棉花没有作用。3. 这些成分共同作用可将棉花完全转化为水溶性产物，但分离后会失去这种能力，按原始比例重新组合后又能定量恢复该能力。
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