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Biochem J. 1967 Sep;104(3):716-24. doi: 10.1042/bj1040716.
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9
Cellulolytic enzyme system of Trichoderma koningii. Separation of components attacking native cotton.康宁木霉的纤维素分解酶系统。攻击天然棉花的组分的分离。
Biochem J. 1968 Sep;109(2):217-27. doi: 10.1042/bj1090217.
10
The formation of short fibres from native cellulose by components of Trichoderma koningii cellulase.康宁木霉纤维素酶组分将天然纤维素转化为短纤维。
Biochem J. 1970 Jan;116(1):35-42. doi: 10.1042/bj1160035.

本文引用的文献

1
The degradation of cotton cellulose by the extracellular cellulase of Myrothecium verrucaria. 2. The existence of an ;exhaustible' cellulase.疣孢漆斑菌胞外纤维素酶对棉花纤维素的降解。2. “可耗尽”纤维素酶的存在。
Biochem J. 1963 Aug;88(2):288-96. doi: 10.1042/bj0880288.
2
The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis.可溶性纤维素衍生物的生物降解及其与纤维素水解机制的关系。
J Bacteriol. 1950 Apr;59(4):485-97. doi: 10.1128/jb.59.4.485-497.1950.
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THE FRACTIONATION OF MYROTHECIUM VERRUCARIA CELLULASE BY GEL FILTRATION.通过凝胶过滤法对疣孢漆斑菌纤维素酶进行分级分离。
Biochem J. 1965 Mar;94(3):578-83. doi: 10.1042/bj0940578.
4
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
5
PURIFICATION AND CHARACTERIZATION OF TWO TYPES OF CELLULASE FROM TRICHODERMA KONINGI.康宁木霉两种纤维素酶的纯化与特性分析
J Biochem. 1964 Feb;55:209-12.
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A RAPID METHOD OF DISK ELECTROPHORESIS.一种快速的圆盘电泳法。
Nature. 1963 Jul 13;199:179-80. doi: 10.1038/199179b0.
7
Recycling chromatography.循环色谱法。
Arch Biochem Biophys. 1962 Sep;Suppl 1:152-6.
8
The degradation of cotton cellulose by the extracellular cellulas cellulase of Myrothecium verrucaria.疣孢漆斑菌的胞外纤维素酶对棉花纤维素的降解作用。
Biochem J. 1961 Jun;79(3):562-6. doi: 10.1042/bj0790562.
9
Serum glyco protein concentrations in experimental tuberculosis of guinea pigs.豚鼠实验性结核病中的血清糖蛋白浓度
Am Rev Tuberc. 1953 Oct;68(4):594-602. doi: 10.1164/art.1953.68.4.594.
10
Individual roles of cellulase components derived from Trichoderma viride.源自绿色木霉的纤维素酶组分的各自作用。
Arch Biochem Biophys. 1965 Aug;111(2):439-47. doi: 10.1016/0003-9861(65)90207-9.

绿色木霉的纤维素酶。参与棉纤维溶解的成分的分离。

The cellulase of Trichoderma viride. Separation of the components involved in the solubilization of cotton.

作者信息

Selby K, Maitland C C

出版信息

Biochem J. 1967 Sep;104(3):716-24. doi: 10.1042/bj1040716.

DOI:10.1042/bj1040716
PMID:6069203
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1271210/
Abstract
  1. Culture filtrates from Trichoderma viride have been fractionated by gel filtration on Sephadex G-75 followed by ion-exchange chromatography on DEAE- and SE-Sephadex. 2. The components essential for attack on cotton are a carboxymethylcellulase, a cellobiase and a third (C(1)) component which has no action on CM-cellulose, cellobiose or cotton. 3. These components, which together can completely convert cotton into water-soluble products, lose this ability when separated and regain it quantitatively when recombined in their original proportions.
摘要
  1. 绿色木霉的培养滤液先用葡聚糖凝胶G-75进行凝胶过滤，然后在DEAE-葡聚糖凝胶和SE-葡聚糖凝胶上进行离子交换色谱分离。2. 攻击棉花所必需的成分是一种羧甲基纤维素酶、一种纤维二糖酶和第三种（C(1)）成分，该成分对羧甲基纤维素、纤维二糖或棉花没有作用。3. 这些成分共同作用可将棉花完全转化为水溶性产物，但分离后会失去这种能力，按原始比例重新组合后又能定量恢复该能力。