Kuhad R C, Kumar M, Singh A
Department of Microbiology, University of Delhi South Campus, New Delhi, India.
Lett Appl Microbiol. 1994 Nov;19(5):397-400. doi: 10.1111/j.1472-765x.1994.tb00486.x.
Multiple mutagenesis of Fusarium oxysporum DSM 841 resulted in enhanced yields of cellulases. The hypercellulolytic mutant (NTG-19) secretes high levels of extracellular cellulases on different cellulosic substrates. Addition of surfactant, Tween-80, further increased enzyme secretion by about 30%. The results on hydrolysis of wheat straw by parent strain, DSM 841 and mutant NTG-19 cellulases also revealed a significant improvement in the hydrolytic potential of the cellulolytic enzymes from the mutant NTG-19.
