Sarkaki Alireza, Rezaiei Moslem, Gharib Naseri Mohammadkazem, Rafieirad Maryam
Physiology Research Center and Medicinal Plants Research Center, Ahvaz Jundishapur University of Medical Sciences, 61357-19754 Ahvaz, Iran.
Malays J Med Sci. 2013 Mar;20(2):25-34.
This study aimed to evaluate the effect of two weeks oral administration of pomegranate seed extract (PGSE) on active and passive avoidance memories after permanent bilateral common carotid arteries occlusion (2CCAO) to induce permanent cerebral ischemia in adult female rats.
Seventy adult female Wistar rats (250 ± 20 g) were used. Animals were divided randomly into seven groups with 10 in each: 1) Sham-operated; 2) Ischemic; 3-6) Ischemic received PGSE (100, 200, 400, and 800 mg/2mL/kg, orally) for 14 days; 7) Ischemic received vehicle. In order to create 2CCAO, carotid arteries were ligatured and then cut bilaterally. Active and passive avoidance task were measured using criterion condition responses (CCRs) in Y-maze and step-through latency (STL) in two-way shuttle box in all female rats.
Both active and passive avoidance memories were significantly impaired in rats after cerebral hypoxia-ischemia (CHI) (P < 0.001). PGSE treatment significantly improved passive and active memory impairments with 2CCAO (P < 0.05, P < 0.01, and P < 0.001). No toxicity was observed even with high-dose PGSE consumption (800 mg/kg, for 14 days).
PGSE exhibits therapeutic potential for avoidance memories, which is most likely related at least in part to its antioxidative and free radical scavenging actions.
本研究旨在评估成年雌性大鼠永久性双侧颈总动脉闭塞（2CCAO）诱导永久性脑缺血后，口服石榴籽提取物（PGSE）两周对主动和被动回避记忆的影响。
使用70只成年雌性Wistar大鼠（250±20克）。动物被随机分为七组，每组10只：1）假手术组；2）缺血组；3 - 6）缺血组口服PGSE（100、200、400和800毫克/2毫升/千克），持续14天；7）缺血组给予赋形剂。为了造成2CCAO，双侧结扎并切断颈动脉。在所有雌性大鼠中，使用Y迷宫中的标准条件反应（CCRs）和双向穿梭箱中的穿梭潜伏期（STL）测量主动和被动回避任务。
脑缺氧缺血（CHI）后大鼠的主动和被动回避记忆均显著受损（P<0.001）。PGSE治疗显著改善了2CCAO引起的被动和主动记忆损伤（P<0.05、P<0.01和P<0.001）。即使高剂量服用PGSE（800毫克/千克，持续14天）也未观察到毒性。
PGSE对回避记忆具有治疗潜力，这很可能至少部分与其抗氧化和清除自由基的作用有关。
